Pilates mat exercises and supplementation as strategies to promote health in women 50+

Mat pilates and supplementation as strategy for promoting well-being in women aged 50+: a randomized placebo-controlled clinical trial

Status

Active, not recruiting

Phases

Phase 1

Study type

Interventional

Source

REBEC

Registry ID

RBR-7bwtfry

Enrollment

Registered

2026-04-24

Start date

2026-05-04

Completion date

Unknown

Last updated

2026-04-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Menopause

Interventions

This is a phase 1, randomized, placebo-controlled, prospective, two-arm parallel clinical trial, conducted in a double-blind, third-person blinding design, which aims to evaluate the effects of Mat Pi

Eligibility

Sex/Gender

Female

Age

50 Years to No maximum

Inclusion criteria

Inclusion criteria: To participate voluntarily in the study, participants must: understand the study procedures and agree to participate voluntarily and sign the Informed Consent Form; be 50 years of age or older; be menopausal (12 months or more of amenorrhea); be willing to use the new supplementation with annatto (Bixa orellana L), yerba mate (Ilex paraguariensis A. ST.-Hil.) and hops (Humulus lupulus L.) throughout the study; not be using hormone replacement therapy; and have a skin phototype between I and IV on the Fitzpatrick scale (MSD Manuals Professional Edition, [n.d.]), since the equipment used for cutaneous autofluorescence analysis has adequate sensitivity only for these phototypes, ensuring the reliability of the measurements

Exclusion criteria

Exclusion criteria: Uncontrolled systemic arterial hypertension, having had or currently having cancer, autoimmune diseases such as lupus, inflammatory diseases such as Crohn's disease, no self-reported serious illness, severely ill individuals, and withdrawal of consent; surgery requiring hospitalization within 3 months of the first study visit after screening; absence of health conditions that prevent compliance with study requirements as judged by the investigator based on medical history; severe trauma or surgical event within 3 months of screening; individuals using anticoagulants, since hops have an antiplatelet effect; reluctance or inability to comply with experimental procedures

Design outcomes

Primary

Measure	Time frame
To verify the impact of Mat Pilates practice, associated or not with the use of a compound containing annatto, yerba mate, and hops, on biomarkers, quality of life (QoL), cognitive function, menopausal symptoms, and strength in menopausal women aged = 50 years, over a period of 3 months	—

Secondary

Measure	Time frame
Evaluate advanced glycation end products (AGEs) using skin autofluorescence (SAF) and homocysteine ??levels, which will be assessed by a contracted laboratory using peripheral blood samples, both before and after the interventions;Evaluating Oxidative Stress (OS) before and after the interventions will be carried out in the pharmacy course laboratory at the University of Passo Fundo. The assays performed to evaluate oxidative stress markers are: non-protein thiols (glutathione), nitric oxide, and lipid peroxidation in plasma. For the determination of non-protein thiols, and indirectly of reduced glutathione (the enzyme responsible for the reduction of GSSG to GSH in tissues), the assay described by Ellman (1959) will be used in plasma by measuring a product that reacts with 10 mM DTNB, and reading at 412 nm. For the determination of nitric oxide, the Griess reaction will be performed using a sodium nitrite standard curve as described by Bracht and Ishii-Iwamato (2003). In the evaluation of lipid peroxidation, the amount of malondialdehyde derived from lipid peroxidation will be measured (thiobarbituric acid reactive substances – TBARS – are measured) by heating the biological material to be tested at 100 °C for 15 minutes in the presence of thiobarbituric acid, under acidic conditions, measuring the formation of a color product spectrophotometrically at 535 nm (Ohkawa; Ohishi; Yagi, 1979). All these results will be normalized with the protein content determined by the Lowry method;Evaluate blood count and platelet count before and after the procedures; this will be performed by a contracted laboratory through peripheral blood collection;Evaluate fasting blood glucose levels before and after the interventions; this will be performed by a contracted laboratory through peripheral blood collection;Evaluate the lipid profile (total cholesterol, triglycerides, HDL, LDL, and VLDL) before and after the interventions; this will be performed by a contracted laboratory through	—

Measure

Time frame

Evaluate advanced glycation end products (AGEs) using skin autofluorescence (SAF) and homocysteine ??levels, which will be assessed by a contracted laboratory using peripheral blood samples, both before and after the interventions;Evaluating Oxidative Stress (OS) before and after the interventions will be carried out in the pharmacy course laboratory at the University of Passo Fundo. The assays performed to evaluate oxidative stress markers are: non-protein thiols (glutathione), nitric oxide, and lipid peroxidation in plasma. For the determination of non-protein thiols, and indirectly of reduced glutathione (the enzyme responsible for the reduction of GSSG to GSH in tissues), the assay described by Ellman (1959) will be used in plasma by measuring a product that reacts with 10 mM DTNB, and reading at 412 nm. For the determination of nitric oxide, the Griess reaction will be performed using a sodium nitrite standard curve as described by Bracht and Ishii-Iwamato (2003). In the evaluation of lipid peroxidation, the amount of malondialdehyde derived from lipid peroxidation will be measured (thiobarbituric acid reactive substances – TBARS – are measured) by heating the biological material to be tested at 100 °C for 15 minutes in the presence of thiobarbituric acid, under acidic conditions, measuring the formation of a color product spectrophotometrically at 535 nm (Ohkawa; Ohishi; Yagi, 1979). All these results will be normalized with the protein content determined by the Lowry method;Evaluate blood count and platelet count before and after the procedures; this will be performed by a contracted laboratory through peripheral blood collection;Evaluate fasting blood glucose levels before and after the interventions; this will be performed by a contracted laboratory through peripheral blood collection;Evaluate the lipid profile (total cholesterol, triglycerides, HDL, LDL, and VLDL) before and after the interventions; this will be performed by a contracted laboratory through

—

Countries

Contacts

Public ContactLia Hausen

Universidade de Passo Fundo

prof.liahausen@gmail.com+55(54)999544275

Outcome results

None listed

Source: REBEC (via WHO ICTRP) · Data processed: May 1, 2026