Crohn&apos
Conditions
Interventions
This is a cross-sectional observational study with 80 patients, 30 with Crohn&apos
s disease for collection of samples of intestinal mucosa and fat and mesenteric lymph node
25 control patients who underwent ileocolonoscopy for biopsy of the intestinal mucosa of the terminal ileum
25 control patients who underwent surgery for non inflammatory diseases of the left colon for fat biopsy of normal terminal ileum and mesenteric lymph node. In addition to collection of fecal and peri
s disease, comparing it to the respective controls, aiming at a better molecular knowledge of the disease, in addition to creating bases for the development of biomarkers and potential therapeutic tar
Sponsors
Hospital de Clínicas - Universidade Estadual de Campinas (UNICAMP)
Hospital de Clínicas - Universidade Estadual de Campinas (UNICAMP)
Eligibility
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Patients with endoscopic and histological diagnosis of ileocecal Crohn's disease undergoing surgical procedure. For the control group of distal ileum, patients who underwent colonoscopy for other reasons, whose examination comes without endoscopic changes. For the control group of mesenteric adipose tissue (intestinal fat), patients with non inflammatory diseases of the left colon undergoing surgery, with normal terminal ileum. All survey participants are over 18 years of age
Exclusion criteria
Exclusion criteria: The patients participating in the control groups without the use of medication. And Crohn's patients with disease locations other than the small intestine
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Expected outcome presentation 1: It is expected to identify the transcriptional profile of patients with active Crohn's disease, verified through sequencing analyzes and biological validation, based on the finding of modulation of transcript levels;Outcome presentation 1: Four patients with Crohn's disease who presented with ileal ulcers confirmed by endoscopy and histology were included in the study. The patients had a median disease duration of 11.5 years and a median Crohn's Disease Activity Index (CDAI) of 276.7. All patients underwent surgery due to complications associated with disease progression. Control samples were obtained from eight individuals who underwent bowel surgery for conditions unrelated to inflammatory bowel disease. These control individuals did not present with endoscopic, histological, or clinical signs of IBD. No significant differences were observed between the study groups in anthropometric, nutritional, hematological, or blood biochemical parameters, except for the waist-to-hip ratio, which was higher in the control group compared to patients with Crohn's disease. Total transcriptional analysis identified markers of T-cell signaling pathways in mesenteric adipose tissue associated with Crohn's disease. | — |
Secondary
| Measure | Time frame |
|---|---|
| Expected outcome presentation 2: It is expected to find a significant alteration in the transcriptional signature of regulatory T cells in the mesentery, intestine and blood of patients with Crohn's disease, verified through sequencing analyzes and biological validation, from the levels of transcripts;Outcome presentation 2: Patients with Crohn's disease presented a specific profile of differentially expressed regulatory T cells in mesenteric and intestinal adipose tissues. | — |
Countries
BR
Contacts
Public ContactRaquel Leal
Universidade Estadual de Campinas
Outcome results
None listed