Occupational exposure, waste anesthetic gases, genotoxicity, oxidative stress
Conditions
Interventions
Study group: 60 healthy, young medical residents of both genders. Experimental group: 30 medical residents of anesthesiology and surgery areas with three years of exposure to waste anesthetic gases (i
Other
E05.393.560
E01.370.225.124.200
Sponsors
Faculdade de Medicina de Botucatu - UNESP
Faculdade de Medicina de Botucatu - UNESP
Eligibility
Age
25 Years to 35 Years
Inclusion criteria
Inclusion criteria: Medical residents; young; 25 to 35 years of age; healthy; of both genders; with or without exposure to waste anesthetic gases.
Exclusion criteria
Exclusion criteria: Medical residents with neoclassic disease; inflammatory or infectious conditions; smokers; alcoholics; history of drug abuse; use of medication or antioxidant supplementation; recent exposure to radiation.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The experimental group exposed to waste anesthetic gases will have a significant increase of DNA damage, measured by the Comet Assay, in which is expected to find a minimal increase of 10% in damaged lymphocytes per 100 cells in the exposed group opposed to the control group. | — |
Secondary
| Measure | Time frame |
|---|---|
| The experimental group is expected to show an increase of inflammatory status compared to the control group (increase in 5 to 10% in pro-inflammatory cytokines), measured by the concentration of cytokines in plasma using flow cytometry.;The surgical rooms with air scavenging system are expected to have reduced concentration of waste anesthetic gases then the rooms without a scavenging system, measured with a infrared portable spectophotometer. These values are expected to be below the limits proposed by the National Institute of Occupational Safety and Health (NIOSH, 1994), which are 25ppm for N2O, 2ppm for halogenated agents and 0,5ppm for halogenated agents used with N2O.;The experimental group exposed to waste anesthetic gases will have a significant increase of 7-10% in the concentration of Malondialdehyde (MDA) measured through High-performance liquid chromatography (HPLC) in contrast with the control group, which indicates a higher oxidative status in the exposed group. | — |
Countries
Brazil
Contacts
Public ContactLorena Lucio
Faculdade de Medicina de Botucatu - UNESP
Outcome results
None listed