Genotoxicity, oxidative stress, gene expression, polymorphism, evaluation of lipophilic antioxidants and vitamins
Conditions
Interventions
Sponsors
Departamento de Anestesiologia
Faculdade de Medicina - Unesp Botucatu
Eligibility
Age
18 Years to 50 Years
Inclusion criteria
Inclusion criteria: Healthy individuals who do not present other disease unless the operative condition, undergoing elective ENT and ophthalmic surgery, lasting at least 90 minutes
Exclusion criteria
Exclusion criteria: Smoking, using illicit drugs and alcohol, users of daily vitamin supplements and / or antipyretics, analgesics, antibiotics, anti-inflammatory, among others, the occupationally exposed to paints, fuel, pesticides and hospital environments, those who received radiation ionizing or MRI done in less than 30 days, those who have already undergone chemotherapy or radiotherapy, patients with diseases such as asthma, hypertension, diabetes, cancer, infectious or inflammatory processes, and those who are not referred for surgery of ENT and ophthalmology clinic.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| It is expected to find increased DNA damage in patients of both groups, particularly in the group associated with anesthesia with nitrous oxide, but with greater damage to collect M1 (90 minutes after the initiation of surgery) using the comet assay in lymphocytes, image analyzed by fluorescence microscopy with the aid of a software capable of detecting the intensity of the tail, and the tail greater, the greater the damage. Increased oxidative stress by the test carbonyls and malondialdehyde protein. And the reduction of TAP test (total antioxidant capacity) performed in serum or plasma. There may be some imbalance of antioxidants and vitamins, so it is necessary to evaluate these antioxidants and vitamins to better elucidate the effect of desflurane associated or not with nitrous oxide in the patient's body. The results of gene expression and polymorphism will also give a direction of the effects of these anesthetics. The results obtained are comparable between groups and times to check significant difference. | — |
Secondary
| Measure | Time frame |
|---|---|
| Are not expected | — |
Countries
Brazil
Contacts
Public ContactFlávia Nogueira
Faculdade de Medicina - Unesp Botucatu
Outcome results
None listed