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Study of occupational exposure in anesthesiologists

Evaluation of redox status, inflammatory response, telomeres and gene expression in anesthesiologists

Status
Active, not recruiting
Phases
Unknown
Study type
Observational
Source
REBEC
Registry ID
RBR-6gf6dx
Enrollment
Unknown
Registered
2017-09-12
Start date
2017-03-01
Completion date
Unknown
Last updated
2025-10-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Oxidative Stress

Interventions

There will be no intervention. Cryopreserved samples of anesthesiologists and physicians not occupationally exposed will be used in continuation of a previously developed study (Evaluation of DNA dama
Other
E01.370.225.998.110

Sponsors

Departamento de Anestesiologia da Faculdade de Medicina de Botucatu
Lead Sponsor
Faculdade de Medicina de Botucatu
Collaborator

Eligibility

Age
23 Years to 75 Years

Inclusion criteria

Inclusion criteria: Anesthesiologists to compose the exposed group medical and clinical settings to compose the control group; healthy volunteers; both genders; non-smoking; age between 23 to 75 years.

Exclusion criteria

Exclusion criteria: Malignants diseases; infectious diseases; inflammatory diseases; alcohol consumption; use of tobacco; illicit drug use; use of medications; use of vitamin supplementation; radiation exposure.

Design outcomes

Primary

MeasureTime frame
To evaluation of oxidative stress will be analyzed oxidative damages in the genome (comet assay), lipid peroxidation (malonaldehyde), total antioxidant capacity (TAP), and nitric oxide (evaluated by the concentrations of nitrite and nitrate) and the various individual antioxidants, homocysteine ??and folate, which will be analyzed by chemiluminescence. The inflammatory response will be assessed by pro-inflammatory cytokines (IL-6, IL-8 and IL-17) by flow cytometry, determination of high sensitivity C-reactive protein will be performed by chemiluminescent immunoassay and liver enzymes alanine transaminase ( ALT) and aspartate transaminase (AST) will be detected in the serum by means of dry chemistry. Expression of two DNA-repair genes (hOGG1 and XRCC1) and three inflammation-related genes (IL-6, IL-8 and IL-17) will be evaluated by quantitative real-time PCR (qPCR). This methodology will also be used to evaluate telomere length.—

Secondary

MeasureTime frame
Are not expected.—

Countries

Brazil

Contacts

Public ContactKátina de Souza

Faculdade de Medicina de Botucatu

katinameneghetti@gmail.com5514997933860

Outcome results

None listed

Source: REBEC (via WHO ICTRP)