Obesity
Conditions
Interventions
The study will be divided into two stages. In step 1 a pilot study will be carried out with 6 patients with obesity without comorbidities and a sequential crossover design, with the objective of knowi
M2: High fat diet intake followed by green tea or placebo,
AFTER A TWO WEEK WASHOUT PERIOD. From the response obtained in step 1, the sample size and the best collection time after the high fat meal
Dietary supplement
Sponsors
Faculdade de Medicina de Botucatu- Unesp
Faculdade de Medicina de Botucatu- Unesp
Eligibility
Age
18 Years to 65 Years
Inclusion criteria
Inclusion criteria: age greater than or equal to eighteen years and obesity (BMI greater than or equal to thirty kilograms per square meter).
Exclusion criteria
Exclusion criteria: Use of dietary supplements and systemic corticosteroids in the last three months, diagnosis of chronic non transmissible diseases such as hypertension, diabetes mellitus II, dyslipidemia, thyroid disease, neoplasms, coronary artery disease, stroke, heart failure, renal or hepatic failure and allergy to food offered in the survey.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Acute green tea supplementation reduces the inflammatory process (interleukin 6, TNFalpha and C-reactive protein, evaluated through commercially available kits and using the ELISA technique), reduce oxidative stress (concentrations of carbonyl groups based on the reaction with dinitrophenylhydralazine and the formation of the Schiff bases and malondialdehyde by high performance liquid chromatography) and modulate the expression of microRNAs (global expression performed through the ManqrayRodentMicroRNACards (A + B card sets v3.0) (Life Technologies) Taq platform). | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary outcomes are not expected | — |
Countries
Brazil
Contacts
Public ContactRodrigo Bastos
Rodrigo Velloni da Silva Bastos
Outcome results
None listed