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Identification of fungi in users of dentures by cheap molecular methods

Identification of Candida spp. in users of removable dentures by low-cost molecular methods

Status
Recruiting
Phases
Unknown
Study type
Observational
Source
REBEC
Registry ID
RBR-5zkjsgj
Enrollment
Unknown
Registered
2022-06-09
Start date
2021-06-15
Completion date
Unknown
Last updated
2025-10-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Denture stomatitis

Interventions

The objective of this study will be to validate the qPCR method with analysis of the dissociation curve and PCR/RFLP for the identification of Candida species isolated from patients using dentures and
E01.370

Sponsors

Universidade Estadual Paulista Julio Mesquita Filho
Lead Sponsor
Universidade Estadual Paulista Julio Mesquita Filho
Collaborator

Eligibility

Age
30 Years to 90 Years

Inclusion criteria

Inclusion criteria: Volunteers of both sexes; from 30 to 90 years; upper complete denture wearers; with or without prosthetic stomatitis

Exclusion criteria

Exclusion criteria: Use of antibiotics; antifungals in the last 3 months

Design outcomes

Primary

MeasureTime frame
Identification of Candida, using molecular methods (PCR with analysis of the dissociation curve and PCR/RFLP), in patients using removable dentures—

Secondary

MeasureTime frame
Candida quantification, patient samples will be plated on Sabouraud Dextrose Agar and CHROMAgar Candida media, for quantification and presumptive identification, respectively, of Candida spp.—

Countries

Brazil

Contacts

Public ContactYuliana Del Pilar Chacón

Universidade Estadual Paulista Julio Mesquita Filho

yuliana.v.chacon@unesp.br+55-16-3301-6557

Outcome results

None listed

Source: REBEC (via WHO ICTRP)