Tooth Loss
Conditions
Interventions
Each treatment modality [proanthocyanidin, naringenin/hesperidin and control group] were performed in 10 patients, randomly assigned to the groups. Patients of the proanthocyanidin group took 300 mg t
Drug
Procedure/surgery
Genetics
SP6.051.227
E06.892
Sponsors
Universidade Federal de Santa Catarina - UFSC
Universidade Federal de Santa Catarina - UFSC
Eligibility
Sex/Gender
Male
Age
21 Years to 70 Years
Inclusion criteria
Inclusion criteria: adult male patients, non-restorable tooth and installation of oral implants; need for implant-supported crown
Exclusion criteria
Exclusion criteria: History of chemotherapy or radiotherapy of the head and neck; previous periodontal disease; alcohol abuse; history of bisphosphonate therapy; diabetic patients who were decompensated; smokers; any contraindication to oral surgery
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Natural products could lead to altered soft and mineralized tissue parameters. 24 hours after extraction, a sample was collected from the socket, RNA was extracted with TRIzol and converted into cDNA to evaluate the effects of two dietary supplements on growth, inflammatory and coagulation factors using qRT-PCR. 60 days after tooth extraction, a bone sample was obtained, fixed in 10% buffered formalin and demineralized by 0.5 M EDTA pH 7.6. Samples were stained in H&E for morphology, PSR to evaluate the organization/maturation of collagen and TRAP for osteoclast counts. Evaluations of growth factors and bone histology will begin within 2 weeks after collection of all samples.;qRT-PCR revealed that the proanthocyanidin group had a statistically significant decrease in the growth factors TGFb-1 (0,61 +- 0,07) and PDGF (0,26 +- 0,07) and in the inflammatory markers IL1-b (0,79 +- 0,17), CXCL2 (0,46 +- 0,07) e IL-6 (0,35 +- 0,1). Naringenin/hesperidin showed an increase in the coagulation factor VWF (2,04 +- 0,44). Histological analyses showed a clear decrease in collagen density (28,8%), bone maturation (66,2%), increased osteoclast density (1,02 + 0,13) and abundant presence of platelets and loose connective tissue in Naringenin/hesperidin group. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary outcomes are not expected | — |
Countries
Brazil
Contacts
Public ContactJosé Souza Júnior
Universidade Federal de Santa Catarina - UFSC
Outcome results
None listed