DNA damage of anesthesiologists.
Conditions
Interventions
Experimental group: 30 medical occupationally exposed to residues of inhaled anesthetics.
Control group: 30 medical nonexposed waste of inhaled anesthetics.
Both groups will be submitted to the collec
Other
E01.370.225.998.110
A10.615.550.599
Sponsors
Departamento de Anestesiologia
Faculdade de Medicina de Botucatu
Eligibility
Age
23 Years to 75 Years
Inclusion criteria
Inclusion criteria: Anesthesiologists to compose the exposed group medical and clinical settings to compose the control group; healthy volunteers; both genders; non-smoking; age between 23 to 75 years.
Exclusion criteria
Exclusion criteria: Malignants diseases; infectious diseases; inflammatory diseases; alcohol consumption; use of tobacco; illicit drug use; use of medications; use of vitamin supplementation; radiation exposure.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| It is expected to find increased DNA damage in the exposed group of participants through the comet assay in lymphocytes analyzed by fluorescence microscopy images with the aid of a software capable of detecting the intensity of the tail, and the higher the tail damage . Increase of cytogenetic damage in the DNA of the exposed participants are also expected by the micronucleus test in the oral mucosa cells, assessed by light microscopy and determined by the presence of micronuclei and nuclear buds by manual counting. The results from both tests are comparative between the exposed and control groups for significant difference check. | — |
Secondary
| Measure | Time frame |
|---|---|
| Are not expected. | — |
Countries
Brazil
Contacts
Public ContactKátina de Souza
Faculdade de Medicina de Botucatu
Outcome results
None listed