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Human semen freezing : Effects of adding lipids and antioxidants in the quality of sperm

Human semen cryopreservation: effect of lipid and antioxidant supplementation on sperm viability

Status
Recruiting
Phases
Unknown
Study type
Interventional
Source
REBEC
Registry ID
RBR-4k5mtn
Enrollment
Unknown
Registered
2016-10-17
Start date
2015-07-20
Completion date
Unknown
Last updated
2025-10-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infertility

Interventions

Intervention group: 63 men with normal semen parameters, evaluated by a sperm. The semen will be divided into two equal volumes, in which half will be frozen with the medium cryoprotectants currently
Other

Sponsors

Faculdade de Medicina de Ribeirão Preto
Lead Sponsor
Invitra
Collaborator

Eligibility

Sex/Gender
Male
Age
18 Years to 40 Years

Inclusion criteria

Inclusion criteria: Men aged 18-40 years; able to collect the semen by masturbation ; are prior to sexual abstinence of at least two days

Exclusion criteria

Exclusion criteria: Semen volume smaller than 1.5 ml; concentration less than 15 million sperm / mL ; motility less than 32 %

Design outcomes

Primary

MeasureTime frame
The frozen semen with the environment cryoprotectants tested in the project has a higher number of spermatozoa with progressive motility, verified by the sperm.;The frozen semen with the environment cryoprotectants tested in the project presents fewer sperm with fragmented DNA, verified by the TUNEL test.

Secondary

MeasureTime frame
The membrane of the sperm frozen with cryoprotectants tested through the project has adhered lipids that come from the environment, checked by scanning electron microscopy.

Countries

Brazil

Contacts

Public ContactRosana dos Reis

Faculdade de Medicina de Ribeirão Preto

romareis@fmrp.usp.br+ 55 (16) 99992 5722

Outcome results

None listed

Source: REBEC (via WHO ICTRP)