aging
Conditions
Interventions
90 elderly at risk of functional decline will be stratified by age and sex and randomized by random drawing in "Excel", constituting two groups.
Experimental group: 45 elderly at risk of functional de
Sponsors
Universidade de Cruz Alta
Universidade regional do noroeste do estado do Rio Grande do Sul
Unimed Planalto Central
Eligibility
Age
60 Years to No maximum
Inclusion criteria
Inclusion criteria: Elderly people included in the Viver 60+ project (screening project for functional decline of users of UNIMED Planalto Centro in partnership with Unicruz). Being a resident of Cruz Alta. Show signs of functional decline. Have been submitted to the pre-test evaluations of the project.
Exclusion criteria
Exclusion criteria: Elderly people who do not accept to participate in the program and those who have medical contraindications or who do not participate in at least 25% of the proposed sessions within the three-month period
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Assess mental health (evidence of depression) trough the questionnaire for screening risk for depression: Geriatric Depression Scale in abbreviated version of Yesavage. The score is considered according to the intensity of depressive symptoms: from 0 - 5 points: normal; 6-10 points: mild depression and from 11 points: severe depression. Test performed before starting and at the end of the 3-month intervention period. The results will be expressed by descriptive statistics and the comparison between pre and post tests will be done through the paired T test (data parametric) or Wilcoxon Test (non-parametric data), considering statistically significant analyzes with p =0.05 | — |
Secondary
| Measure | Time frame |
|---|---|
| Assess DNA damage repair in peripheral blood mononuclear cells through venous blood collection. To determine DNA damage in the mononuclear cells of the elderly, 1 million isolated cells will be immediately labeled with anti-H2Ax and anti-PARP cleaved antibodies. To determine the DNA damage repair capacity, 1 million mononuclear cells will be incubated with 10 µM hydrogen peroxide for 15 minutes at 37ºC. After this incubation, the cells will be immediately labeled with anti-H2Ax and anti-cleaved PARP antibodies. That is, we will have 2 DNA damage reading points: (1) baseline damage, (2) damage after 15 minutes of insult with hydrogen peroxide. The determination of H2Ax and PARP Cleavage markers will be performed by the phosflow technique. To perform this assay, the mononuclear cells will be fixed and permeabilized with Perm/Fix Buffer for 40 minutes at 4 ºC. After fixation and permeabilization, cells will be washed twice with Perm/Wash Buffer and resuspended in 100 µL of the same buffer. To the 100 µL of Perm/Wash Buffer, 5 µL of anti-cleaved PARP antibody and 2 µL of anti-?H2Ax antibody will be added and an incubation period of 60 minutes at 4°C will be performed. Finally, cells will be washed and resuspended in 300 µL Perm/Wash Buffer. Twenty thousand events will be acquired and analyzed in a BD Accuri C6 Plus flow cytometer. Non-labeled and single-marked controls will be used to perform the cytometer set-up and compensation. Data will be expressed as % of positive cells for each marker and as the median of the fluorescence intensity of positive cells for each marker. The evaluation will be carried out before and after the intervention protocol with dance. The results will be expressed by descriptive statistics and the comparison between pre and post tests will be made using the paired T test (parametric data) or Wilcoxon test (nonparametric data), considering statistically significant analyzes with p =0.05;Assess inflammation by collecting peripheral venous blood. | — |
Countries
Brazil
Contacts
Public ContactRaquel Sant'Anna Prates
Universidade de Cruz Alta
Outcome results
None listed