Varicocele
Conditions
Interventions
Experimental group: six men with varicocele have their sperm RNAs sequenced to quantify the gene expression, through large-scale sequencing technique (RNA-seq), divided into two groups: group A, compo
group B, three patients with varicocele grade III with abnormal semen analysis.
Control group: six men without varicoceles will have their sperm sequenced RNAs for quantification of gene expression, t
Group D, three infertile patients.
Genetics
E05.393.760.319
Sponsors
Faculdade de Medicina de Ribeirão Preto
Centro Regional de Hemoterapia de Ribeirão Preto
Eligibility
Sex/Gender
Male
Age
18 Years to 45 Years
Inclusion criteria
Inclusion criteria: Study group: men that have varicocele in grades II and III; aged between 18 and 45 years; not having done previous surgery correction of the disease. Control groups: men aged between 18 and 45 years; fertility proven by having at least two children; and selected men from couples with infertility for male factor or unexplained infertility.
Exclusion criteria
Exclusion criteria: Smoking; excessive use of alcohol; drug abuse; genetic damage; prior varicocele correction surgery; testicular trauma; urogenital infections suspicion; cancer; endocrinopathies; treatments that may influence the testicular functions.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Difference in the profile of transcripts in sperm of patients with varicocele, compared to fertile and infertile men, verified by RNA-seq technique from finding a variation of at least 5% compared between groups. | — |
Secondary
| Measure | Time frame |
|---|---|
| Selection of candidate genes to identify differential gene expression between the groups with and without varicocele by real time PCR methodology based on the statement of a variation of at least 5% compared between groups. | — |
Countries
Brazil
Contacts
Public ContactRosana dos Reis
Faculdade de Medicina de Ribeirão Preto
Outcome results
None listed