Periapical Periodontitis,
Conditions
Interventions
The objective of this clinical study is to investigate the effects of endodontic treatment, using an irrigation protocol, on bacteria, endotoxins and evaluation of cytotoxic effects in root canals wit
EDTA 17% will be used to remove smear layer and Calcium hidroxide solution as final irrigation. The root canals were filled with calcium hydroxide for 14 days and then the canals will be obturated. Sa
after an irrigation prorocol (S3)
after intracanal medication (S4).
Procedure/surgery
E06.397.778
Sponsors
Faculdade de Odontologia da Universidade de São Paulo
Faculdade de Odontologia da Universidade de São Paulo
Eligibility
Age
13 Years to No maximum
Inclusion criteria
Inclusion criteria: Patients of both genders; periapical bone rarefaction visible on radiography; without previous endodontic treatment, under conditions that justify the endodontic treatment; without systemic alterations, such as chronic degenerative diseases, infectious processes, autoimmune diseases or induced or acquired immunodeficiencies; absence of antibiotics in the 90 days prior to the beginning of the research
Exclusion criteria
Exclusion criteria: teeth with a periodontal pocket greater than 4 mm; roots with a length of less than 15 mm or greater than 25 mm; incomplete apicigenesis; loss of dental tissue that prevents absolute isolation, or root canal alterations, calcifications or resorptions.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Reduction of 80% of bacterial levels after the different steps of endodontic treatment, verified by the quantitative PCR method by analyzing rDNA and rRNA. | — |
Secondary
| Measure | Time frame |
|---|---|
| Reduction of 40% of endotoxin levels and neutralization of cytotoxic effects after final irrigation protocol and intracanal medication, verified by LAL and ELISA. | — |
Countries
Brazil
Contacts
Public ContactLais Prado
Faculdade de Odontologia da Universidade de São Paulo
Outcome results
None listed