Occupational Exposure
Conditions
Interventions
This work will evaluate the concentrations of anesthetic gas residues (RGA) isoflurane and sevoflurane in operating rooms.
There will be a single collection of biological material (buccal exfoliative
Other
Sponsors
Faculdade de Medicina de Botucatu - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
Departamento de Anestesiologia
Eligibility
Inclusion criteria
Inclusion criteria: Have at least one year of operation in the surgical center; surgery of small and large animals; animal husbandry; animal reproduction; sádios volunteers; both genders; age between 18 and 60 years
Exclusion criteria
Exclusion criteria: Carriers of malignant disease; infectious diseases: inflammatory; heavy use of alcohol; of "illicit drugs"; medicines; vitamin supplementation; work in some area with occupational exposure
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The expected outcome is an increase in DNA damage. The methods of the comet test will be used to evaluate damages and genetic instability by the micronucleus test (MN) method, and cytotoxicity by flow cytometry, where viability and apoptosis and buccal micronucleus will be analyzed, where genomic instability will also be evaluated. Results that achieve a level of 5% will be considered significant. The sample size was calculated based on previous studies with the MN test assuming an expected mean difference between the groups for the buccal MN test around 0.52 and a standard deviation of 0.56, being determined 30 individuals per group with 95% test power. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary outcomes are not expected | — |
Countries
Brazil
Contacts
Public ContactDrielle Figueiredo
Faculdade de Medicina de Botucatu - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
Outcome results
None listed