Endodontic treatment versus retreatment: inflammatory and microbiological evaluation, before during and after 18 months of treatment

Primary versus secondary endodontic infection: inflammatory and lipid profile, microbial profile, endotoxin and LTA levels before and during endodontic therapy and success after 18 months of treatment

Status

Recruiting

Phases

Phase 2

Study type

Interventional

Source

REBEC

Registry ID

RBR-3xbnnn

Enrollment

Unknown

Registered

2018-07-17

Start date

2017-07-12

Completion date

Unknown

Last updated

2025-10-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dental Pulp Diseases

Interventions

Group 1 Number of participants: 15 Procedures performed: Endodontic treatment Medication: Calcium hydroxide P.A. with serum Availability: In stock Medication time: 14 days Method of administration: in

Drug

Procedure/surgery

E06.397.778

E06.397.778.889

E06.397.778.778

Eligibility

Inclusion criteria

Inclusion criteria: Patients in adulthood; Need for endodontic treatment; Permanent teeth with primary endodontic infection; Unirradicular; Presence of a single canal and periapical lesion; Absence of periodontal pockets greater than 4 mm; No mobility.

Exclusion criteria

Exclusion criteria: Teeth that can't be insulated with the rubber dam; With root fracture; Exposure of the pulp chamber; Patients who have used antibiotics or antifungals in the last 3 months; Patients with systemic disease

Design outcomes

Primary

Measure	Time frame
Expected outcome 1: Increased release of local lipid mediators (RvE1, RvD2 and LxA4) with resolution or significant reduction of the periapical inflammatory process, verified by the enzyme-linked immunosorbent assay (ELISA) method, which allows detecting amounts of protein of nanograms (10-9 g) from the observation of a variation of at least 5% in the pre- and post-intervention measurements;Expected outcome 2: detection and quantification of lipoteichoic acid (LTA), verified by means of the enzyme-linked immunosorbent assay (ELISA) method, which allows the detection of protein amounts of nanograms (10-9 g) from a variation of at least 5% in the pre- and post-intervention measurements	—

Measure

Time frame

Expected outcome 1: Increased release of local lipid mediators (RvE1, RvD2 and LxA4) with resolution or significant reduction of the periapical inflammatory process, verified by the enzyme-linked immunosorbent assay (ELISA) method, which allows detecting amounts of protein of nanograms (10-9 g) from the observation of a variation of at least 5% in the pre- and post-intervention measurements;Expected outcome 2: detection and quantification of lipoteichoic acid (LTA), verified by means of the enzyme-linked immunosorbent assay (ELISA) method, which allows the detection of protein amounts of nanograms (10-9 g) from a variation of at least 5% in the pre- and post-intervention measurements

—

Secondary

Measure	Time frame
Expected outcome 3: Reduction of the number of bacteria present in the root canals in teeth of patients with apical periodontitis compared to biomechanical post-preparation values and intracanal medication, made by counting Colony Forming Units (CFU / ml);Expected outcome 4: Reduction in endotoxin levels by means of the Chromokinetic Test of Limulus Amebocyte Lysates (KQCL) compared to the initial values from the observation of a variation of at least 5% in the pre and post intervention measurements;Expected outcome 5: Evaluation of the volume of the initial periapical lesions and after 18 months of treatment by Cone-Beam Computed Tomography. It is expected to find lower lesion regression in cases with higher endotoxin indices	—

Measure

Time frame

Expected outcome 3: Reduction of the number of bacteria present in the root canals in teeth of patients with apical periodontitis compared to biomechanical post-preparation values and intracanal medication, made by counting Colony Forming Units (CFU / ml);Expected outcome 4: Reduction in endotoxin levels by means of the Chromokinetic Test of Limulus Amebocyte Lysates (KQCL) compared to the initial values from the observation of a variation of at least 5% in the pre and post intervention measurements;Expected outcome 5: Evaluation of the volume of the initial periapical lesions and after 18 months of treatment by Cone-Beam Computed Tomography. It is expected to find lower lesion regression in cases with higher endotoxin indices

—

Countries

Brazil

Contacts

Public ContactBruna Jordão Motta Corazza

Universidade Estadual Paulista Júlio de Mesquita Filho - Campus São José dos Campos

brunajordao1991@gmail.com+55-012-991512822

Outcome results

None listed

Source: REBEC (via WHO ICTRP) · Data processed: Feb 17, 2026