Obesity
Conditions
Interventions
with normal BMI but with periodontitis (NP = 10)
As this is an observational investigation, there is no intervention in this study. The aim of this study was to evaluate the salivary proteomic profile related to obesity and periodontitis in women du
overweight (n = 10)
arterial hypertension during pregnancy (n = 5)
Gestational Diabetes Mellitus (n = 4)
smoker (n = 3)
SARS-CoV-2 diagnosis (n = 3)
multiple tooth loss (n = 2)
twin pregnancy (n = 1)
underweight (n = 1). The follow-up of these women was carried out, at least, 1 month after delivery, between May/2021 and August/2021. The final composition of the sample was pregnant women with obesi
with obesity but without periodontitis (OWP = 10)
with normal BMI and without periodontitis (NWP = 10). Saliva samples were collected and analyzed to investigate proteins associated with obesity and periodontitis. Observational measurements were: ant
Sponsors
Faculdade de Odontologia de Bauru - Universidade de São Paulo
Faculdade de Odontologia de Bauru - Universidade de São Paulo
Eligibility
Sex/Gender
Female
Age
18 Years to 40 Years
Inclusion criteria
Inclusion criteria: Pregnant women aged 18–40 years; who were in the 3rd trimester of pregnancy, between 27th and 39th gestational weeks; with regular follow-up with the obstetrician; and who had adequate cognitive function during pregnancy, without impairments that required absolute rest
Exclusion criteria
Exclusion criteria: Twin pregnancy; individuals with neuromotor impairment; arterial hypertension during pregnancy; Gestational Diabetes Mellitus; malnutrition; overweight, therefore, BMI between 25.00 and 29.99 kg/m2; confirmed or suspected diagnosis of SARS-CoV-2 infection; with hyposalivation; subjects who were using or who used at some point in pregnancy antibiotics or any medication that could interfere with periodontal status and/or salivary flow, e.g., immunosuppressive, anticonvulsant or calcium channel-blocking drugs, such as cyclosporine, phenytoin or nifedipine, respectively; who were under orthodontic treatment or any dental treatment with another professional; participants with cavitated caries lesions; with severe dental wear; with diagnosis of stages I and IV of periodontitis; with multiple tooth loss, being more than two teeth per hemiarch; with self-reported systemic disease besides obesity; and users of alcohol, tobacco, or illicit drugs
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Expected outcome 1. It was expected to find proteins differentially expressed in the saliva of pregnant women with obesity and periodontitis, using a mass spectrometer. Specifically, we expected to find higher levels of inflammatory proteins such as matrix metalloproteinase 9, S100 proteins, complement C3, profilin 1, alpha 2 macroglobulin, haptoglobin, androgen regulated protein of the submaxillary gland, histatin 1, fatty acid binding protein , thioredoxin and albumin;Outcome 1. Through the use of mass spectrometer, differently expressed proteins in saliva were identified when obesity and periodontitis were present in combination in pregnant women, such as submaxillary gland androgen regulated protein 3B, protein S100 A8, matrix metalloproteinase 9, heat shock 70 kDa protein 2 and 6, putative heat shock 70 kDa protein 7, heat shock 71 kDa protein, haptoglobin, plastin-1, prolactin inducible protein and alpha defensins 1 and 3;Expected outcome 2. It was expected to identify using a mass spectrometer a reduction in salivary proteins in women with obesity and periodontitis and women with normal weight and periodontitis after delivery;Outcome 2. Through the use of mass spectrometer, reduction in albumin levels after delivery was identified for all groups. Submaxillary gland androgen regulated protein 3B reduced only in the group of obese women, while it increased in the group of normal weight women. S100-A8 protein expression increased after delivery for women with obesity and periodontitis. Heat shock 70 kDa protein 6 and putative heat shock 70 kDa protein 7 were not present after delivery in saliva of women with obesity and periodontitis. Similarly, heat shock 70 kDa protein 6, putative heat shock 70 kDa protein 7, heat shock 70 kDa protein 2 and heat shock cognate 71 kDa protein were not present in the saliva of women with normal weight and periodontitis after delivery. There was an increase in the levels of cystatin-C, -S, -SA, -SN and lipocalin-1 in pregnant wome | — |
Secondary
| Measure | Time frame |
|---|---|
| No secondary outcomes expected | — |
Countries
Brazil
Contacts
Public ContactGerson Foratori Junior
Faculdade de Odontologia de Bauru - Universidade de São Paulo
Outcome results
None listed