Leprosy
Conditions
Interventions
We used polarized orthogonal light videomicroscopy (Microscan®), Fourier analysis of laser-Doppler signal to study vasomotion, and laser-Doppler flowmetry associated to iontophoresis vasoactive subst
Drug
E01.370.350.515.690
Sponsors
Universidade do Estado do Rio de Janeiro
Fundação Carlos Chagas Filho de Amparo À Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Eligibility
Sex/Gender
Male
Age
20 Years to 60 Years
Inclusion criteria
Inclusion criteria: Male; Be a tuberculoid leprosy patient under treatment; Age between 20 and 60 years; Present tuberculoid lesion in skin of superior and inferior limbs; Do not have hypertension or Diabetes mellitus and other diseases that may alter microcirculatory parameters; Do not be a smoker; Do not present leprosy reaction state at the time of the examination; Ability to follow the given guidelines and attend the evaluations; Phototype I-IV of Fitzpatrick and Sign the informed consent form.
Exclusion criteria
Exclusion criteria: Obesity (BMI> 35); Spot present in another anatomical region other than superior and inferior limbs; Single lesion of small-sized tuberculoid lesion with a recent biopsy scar occupying almost the entire lesion.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| It is expected to show the deterioration of the sympathetic innervation of the microcirculation vessels Mycobacterium leprae infection and to prove the vascular dysautonomia model by denervation by means of significant decrease (p <0.05) of vasomotion sympathetic component at the tuberculoid Leprosy skin lesion in relation to healthy skin (control) in the contralateral limb using the method of spectral analysis of vasomomotion by laser-Doppler flowmetry.;No significant difference (p = 0.6461) was observed between the sympathetic component of vasomotion at the lesion site caused by tuberculoid Leprosy (0.2420 ± 0.0823) compared to healthy skin on the contralateral side (0.2420 ± 0, 0648) of the same patient using the spectral analysis of vasomotion by laser Doppler flowmetry and, therefore, it was evidenced that there was no decrease of the sympathetic activity in the lesion region and consequently vascular dysautonomia by denervation in this group of patients with tuberculoid leprosy. Data are expressed as mean±standard deviation. | — |
Secondary
| Measure | Time frame |
|---|---|
| It is expected to be found a significant reduction (p<0.05)in functional capillary density (number of capillaries with blood flow / mm2) in the cutaneous lesion region of tuberculoid Leprosy in relation to the healthy skin area in the contralateral limb of the same leprosy patient.;It was observed a significant reduction of functional capillary density (number of capillaries with blood flow/mm2) in tuberculoid leprosy skin lesion (12,55[12,14-13,27]) in comparison to healthy skin in the contralateral limb (17,48[17,08-18,42]) of the same tuberculoid leprosy patient (p=0,0021), using polarized orthogonal light videomicroscopy method. Data are expressed as median and interquartile range.;It is expected a significantly lower (p<0.05) endothelium-dependent and independemnt vasodilator response in the lesion caused by tuberculoid leprosy (due to the loss of sympathetic tonus in the arterioles) in this region) in relation to healthy (control) skin on the contralateral limb of the same patient, using the laser-Doppler flowmetry associated to acetylcholine (an endothelium-dependent vasodilator) and sodium nitroprusside (an endothelium-independent vasodilator) iontophoresis method. ;It was not observed any differences in endothelium-dependent and independent vasodilation at sites of skin lesion caused by tuberculoid leprosy in relation to normal skin region in the contralateral limb of the same patient, using laser-Doppler flowmetry associated to acetylcholine (endothelium-dependent vasodilator) and sodium nitroprusside (endothelium-independent vasodilator) iontophoresis. Results: Percentual increase of blood flow after endothelium dependent vasodilator iontophoresis in the tuberculoid Leprosy lesion 242,00[125,40-441,90] and in control skin 190,60[110,00-439,70], p=0,8048. Percentual increase of blood flow after endothelium independent vasodilator intophoresis in the tuberculoid Leprosy lesion 340,00[52,200-1042,05] in control skin 258,50[118,90-340,20], p=0,8048 | — |
Countries
Brazil
Contacts
Public ContactEliete;Lívia Bouskela;Pino
Universidade do Estado do Rio de Janeiro;Universidade do Estado do Rio de Janeiro
Outcome results
None listed