Pulpitis Stomatognathic Diseases
Conditions
Interventions
This is an observational, analytical, comparative, controlled, non-randomized, non-masked clinical study, with a total of 32 participants of both sexes, aged 18 years or older, who meet the inclusion
Sponsors
Faculdade de Odontologia do Campus de Araçatuba da Universidade Estadual Paulista Júlio de Mesquita Filho
Faculdade de Odontologia do Campus de Araçatuba da Universidade Estadual Paulista Júlio de Mesquita Filho
Eligibility
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Systemically healthy individuals; both sexes; minimum age of 18 years; teeth with vital pulp and at least one root canal viable for collection. Normal pulp group composed of patients presenting teeth without clinical symptoms; absence of caries or fractures; normal response to cold sensibility testing. Symptomatic irreversible pulpitis group composed of patients presenting teeth that are sound or with extensive caries; coronal fracture or deep restorations; absence of pulp exposure to the oral cavity; presence of spontaneous or provoked pain
Exclusion criteria
Exclusion criteria: Use of antibiotics in the three months prior to collection; presence of associated periodontal disease; systemic alterations; teeth with coronal destruction that prevents absolute isolation; root canal exposed to the oral cavity; atretic canals that prevent collection; pulp necrosis with or without fistula; signs of dissemination of endodontic infection; diagnosis of reversible pulpitis or asymptomatic irreversible pulpitis; history of previous endodontic intervention
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To qualitatively and quantitatively evaluate the proteomic profile of inflamed dental pulp in comparison with normal dental pulp and the corresponding saliva samples. The outcome will be verified through proteomic analysis using mass spectrometry in a nanoACQUITY UPLC-Xevo QTof MS system (Waters), with protein identification performed using ProteinLynx Global Server (PLGS) software version 3.0 and the Homo sapiens database from the UniProt catalog. Differences in protein expression between groups, as well as differentially expressed proteins related to inflammatory mechanisms and host defense, will be analyzed | — |
Secondary
| Measure | Time frame |
|---|---|
| To evaluate the correlation between proteins expressed in dental pulp and saliva samples from the participants. The outcome will be verified through comparative proteomic analysis and bioinformatics tools. Common or differentially expressed proteins between tissues and fluids will be analyzed;To evaluate potential salivary biomarkers associated with symptomatic irreversible pulpitis. The outcome will be verified using the Enzyme-Linked Immunosorbent Assay (ELISA). Levels of candidate proteins previously identified through proteomic analysis will be analyzed;To evaluate salivary biochemical parameters related to the inflammatory pulp process. The outcome will be verified through laboratory biochemical analyses. Results obtained from participants with symptomatic irreversible pulpitis will be compared with those from participants with normal pulp | — |
Countries
BR
Contacts
Public ContactRogério Jacinto
Faculdade de Odontologia de Araçatuba - Universidade Estadual Paulista
Outcome results
None listed