Muscle Strength
Conditions
Interventions
A clinical, double-blind, randomized, placebo-controlled study will be carried out with 40 male subjects, aged 18 to 40 years. Participants will be divided into two groups in a 1:1 ratio: Omega 3 (5g/
in addition, blood samples will be used to assess the incorporation of fatty acids into the blood
and muscle biopsies will be used for muscle fiber cross-sectional area (fAST) analysis, activation of rotein synthesis pathways, and fatty acid incorporation into myocytes (lipidomic analysis). In add
Sponsors
Universidade Estadual de Campinas
Fundo de apoio ao ensino, pesquisa e extensão da Universidade de Campinas
Eligibility
Sex/Gender
Male
Age
18 Years to 40 Years
Inclusion criteria
Inclusion criteria: Male; 18 to 40 years old; healthy; physically active (according to the International Physical Activity Questionnaire); trained in strength for at least 12 months; who have a 1-Repetition Maximum of at least 2.5 x body weight; and habitual protein consumption = 1.6g/Kg/d
Exclusion criteria
Exclusion criteria: Previous use of anabolic androgenic steroids; current use (in the last 3 months) of ergogenic aids; being engaged in a calorie-restricted diet (> 1 year); medical restriction regarding the practice of physical activity; allergy to omega 3 supplementation or milk protein; any type of chronic disease or orthopedic injury that prevents the practice of strength exercises
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluate maximum strength, using the 1-repetition maximum test method on the legpress 45º device, through changes in the maximum weight (kilograms) carried by the participants;To evaluate body composition, using the BodPod method, through changes in the parameters of body weight (kilograms), lean mass weight (kilograms and percentage) and fat mass weight (kilograms and percentage);To evaluate the area of ??the rectus femoris and vastus lateralis muscles, using the ultrasonography method, through the increase in muscle area in cm² | — |
Secondary
| Measure | Time frame |
|---|---|
| To evaluate the incorporation of omega 3 fatty acids in blood and skeletal muscle cells, using the lipidomic analysis method, through the identification and quantification of lipid molecular species;To evaluate the cross-sectional area of muscle fibers, using the immunofluorescence method, through quantification in µm²;To evaluate the activation of protein synthesis pathways (mTOR and p70s6k), using the western blotting method, through the amount of phosphorylated proteins in response to physical exercise | — |
Countries
Brazil
Contacts
Public ContactHeloisa Castanheira Santo André
Universidade Estadual de Campinas
Outcome results
None listed