Sarcopenia Atrofia Muscular
Conditions
Interventions
A randomized, crossover, placebo-controlled study will be conducted with community-dwelling older adults in Brasília, Brazil. Twenty physically active and 20 sedentary older adults will receive a nitr
Sponsors
Faculdade de Ciências da Saúde da Universidade de Brasília
Universidade de Brasília
Eligibility
Age
60 Years to 75 Years
Inclusion criteria
Inclusion criteria: Elderly who live in Brasilia; age between 60 and 75 years old; both sexes; elderly who accept to participate in the study
Exclusion criteria
Exclusion criteria: Elderly with excessive ingestion of alcoholic beverages; smokers; who use drugs that can block the reduction of nitrate and nitrite to nitric oxide (sexual hormones, antacids, proton pump inhibitors, xanthine oxidase inhibitors); drugs that can enhance nitric oxide effects (phosphodiesterase inhibitors); treatment with anticoagulants and immunosuppressive drugs; elderly who can not exercise; with malignant diseases; vegetarian; who do not like or who are advised to not consume green vegetables; who use mouthwashs; who use antibiotics until 7 days before the beginning of the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Expected Outcome 1 - Six-minute walk test: Increase in walking distance (in meters) after the nitrate-rich diet period, assessed using the six-minute walk test, based on the detection of a variation between intervention and baseline periods with a p-value < 0.05.;Observed Outcome 1 - Six-minute walk test: A significant increase in walking distance in the six-minute walk test was observed after the nitrate-rich diet period (p < 0.001).;Expected Outcome 2 - Serum total antioxidant capacity (sTAC / FRAP): Serum total antioxidant capacity (sTAC), assessed using the ferric reducing ability of plasma (FRAP) assay, expressed in µmol/L, measured before and after each 7-day dietary intervention period, based on the detection of a variation between intervention and baseline periods with a p-value < 0.05. This outcome constitutes the primary outcome of the biochemical axis of this clinical trial and served as the basis for the sample size calculation for biochemical analyses. An amendment was submitted prior to manuscript submission to correct an administrative omission in the original trial registration.;Observed Outcome 2 - Serum total antioxidant capacity (sTAC / FRAP): For serum total antioxidant capacity (sTAC), no significant difference was observed between the nitrate-rich and nitrate-poor interventions; however, both showed a significant increase compared with baseline (p < 0.02). | — |
Secondary
| Measure | Time frame |
|---|---|
| Expected outcome 3: A reduction in inflammatory response markers after the nitrate-rich diet period, assessed using commercial enzyme-linked immunosorbent assay (ELISA) kits, based on the detection of a significant change between the intervention and baseline periods with a p-value < 0.05.;Observed outcome 3: No statistically significant differences were observed between the nitrate-rich and nitrate-poor interventions for the inflammatory markers assessed (IL-6 and IL-10) (p > 0.05).;Expected outcome 4: A reduction in the time spent (in seconds) to complete five consecutive repetitions in the five-times sit-to-stand test after the nitrate-rich diet period, confirmed by a statistically significant change between the intervention and baseline periods (p < 0.05).;Observed outcome 4: A significant reduction in time was observed in the five-times sit-to-stand test after the nitrate-rich diet period, indicating improved functional performance (p < 0.001).;Expected outcome 5: An increase in strength (in kilograms) after the nitrate-rich diet period, assessed using the handgrip strength test, based on the detection of a statistically significant change between the intervention and baseline periods (p < 0.05).;Observed outcome 5: A significant increase in handgrip strength was observed after the nitrate-rich diet period (p < 0.001).;Expected outcome 6: A reduction in the time spent (in seconds) to complete the movement after the nitrate-rich diet period, assessed by isolated gait speed and under dual-task conditions (cognitive and motor) over a 10-meter walkway, based on the detection of a statistically significant change between the intervention and baseline periods (p < 0.05).;Observed outcome 6: A significant improvement in mobility was observed after the nitrate-rich diet period, evidenced by increased gait speed under single-task and dual-task/multitask conditions (p < 0.001).;Expected outcome 7: An improvement in cognitive performance after the | — |
Countries
BR
Contacts
Public ContactClíslian da Silva
Faculdade de Ciências da Saúde da Universidade de Brasília
Outcome results
None listed