Blood transfusion reactions
Conditions
Interventions
Assay of a panel of cytokines in plasma and RBC of leucofiltred donated blood
assay of proinflammatory cytokines in plasma and RBCs of non leucofiltred donated blood
Sponsors
A lAzhar University hospital
South Egypt Cancer Institute
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Age: 18-60 years Weight :> 50 kg. Pulse: Regular rhythm (60-100 beats/minute). Blood pressure: 90/60 up to 180/100. Hemoglobin level: (13 - 18 g/dl) for male donors and (12 - 16 g/dl) for female donors. Blood donation interval: 90 days for males and 120 days for females.
Exclusion criteria
Exclusion criteria: Age: 60 years Weight :180/100. Hemoglobin level: 18 g/dl for male donors and 16 g/dl for female donors. Blood donation interval: 90 days for males and 120 days for females.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To investigate the presence of the following panel of cytokines: Macrophage migration inhibitory factor (MIF), Interleukin- 16(IL-16), Hepatocyte growth factor (HGF), Interleukin- 17 (IL-17A), Granulocyte-macrophage colony-stimulating factor (GM-CSF) and Tumor Necrosis Factor Alpha (TNF-a), in red blood cells (RBCs) and the plasma of whole blood units. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1- To assess the effect of storage on the plasma cytokines level, 2- To assess the effect of pre-storage leucofilteration of whole blood on the accumulation of that panel of plasma cytokines during storage, 3- To investigate the possible release of red cell cytokines to the plasma of whole blood units by hemolysis during storage. | — |
Countries
Egypt
Contacts
Public ContactRania Bakry
Professor of Clinical pathology South Egypt cancer institute assiut egypt
Outcome results
None listed