Kidney transplantation, Renal transplantation, Post-kidney transplant state, Solid organ transplantation (kidney), Organ transplantation (renal), Post-transplant immunosuppression, Tacrolimus Kidney transplantation, new kidney, organ transplantation, immune system, tacrolimus
Conditions
Interventions
This study investigates whether the previously developed Ficoll-paque density centrifugation and Automacs isolation procedures can be replaced by the much more time-efficient Easysep isolation, which
Sponsors
Erasmus MC, Universitair Medisch Centrum Rotterdam
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Tacrolimus-Prograf® (twice-daily tacrolimus formulation)-based immunosuppressive regimen Written informed consent Patient is not receiving T-cel depleting antibody therapy with Rabbit anti-thymocyte globulin (rATG) or alemtuzumab or has a T-cel count of at least >200 x 10^6/L.
Exclusion criteria
Exclusion criteria: Age <18y Recipients of a non-renal organ transplant at the same occasion.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The proportion of intra-T lymphocyte tacrolimus measurements obtained with the EasySep ® isolation method that deviate by >15% from the measurements obtained with the reference method (Ficoll-Paque density centrifugation and Automacs). | — |
Secondary
| Measure | Time frame |
|---|---|
| 1) To optimize the timing of the addition of the P-gp blocking drug verapamil and 2) to evaluate the influence of patient characteristics on the whole blood to intraT lymphocyte concentration ratio at two timepoints after dose administration. | — |
Countries
Netherlands
Contacts
Public ContactL. Mingels
Erasmus MC, Universitair Medisch Centrum Rotterdam
Outcome results
None listed