Skeletal muscle atrophy Muscle loss
Conditions
Interventions
All participants will undergo 5 days of unilateral leg immobilization using a knee brace that prevents voluntary quadriceps contraction. In the neutral group, the leg is fixed in full extension (0° fl
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: In order to be eligible to participate in this study, a participant must meet all of the following criteria:Healthy males and femalesAged from 18-40 years at the time of signing informed consent18.5 < BMI < 30 kg·m-2Must be willing and able to communicate and participate in the whole study
Exclusion criteria
Exclusion criteria: A potential participant who meets any of the following criteria will be excluded from participation in this study:SmokingDiabetes (Type 1, Type 2, or genetic form of diabetes)Any diagnosed cardiovascular (heart) disease or high blood pressure (=140 mmHg systolic and/or =90 mmHg diastolic)Chronic use of any prescribed or over the counter pharmaceuticals that may modulate muscle protein metabolism (excluding oral contraceptives and contraceptive devices).A personal or family history of thrombosis, epilepsy, seizures or schizophrenia. Prone to keloid forming (i.e. hyperplastic growth of scars).Any known disorders in muscle metabolismRegular use of dietary protein and/or amino acid supplements (>3 times per week)Currently involved in a structured progressive resistance training programme (>3 times per week)Known allergy to lidocaineKnown severe kidney problemsAllergy to one or multiple amino acidsRecent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture)Having received or ingested a stable isotope tracer containing 15N in the pastContra-indications for MRI such as incompatible metal objects in the body and claustrophobia.Currently taking part in other scientific researchPregnant or breastfeedingUnable to give consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary study endpoint is muscle protein net balance (MPNB), which is calculated by subtracting muscle protein breakdown (MPB) from muscle protein synthesis (MPS). | — |
Secondary
| Measure | Time frame |
|---|---|
| … | — |
Countries
Netherlands
Contacts
Public ContactM.L. Dirks
Wageningen Universiteit
Outcome results
None listed