Not applicable Not applicable
Conditions
Interventions
All subjects will perform a single bout of unilateral lower limb resistance-type exercise. 
Subsequently, participants will be randomized to ingest either 1x 100 g, 4x 25 g, or 15x 
~7 g of protein, provided in the form of calcium caseinate powder.
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: Aged between 18-40 years Healthy18.5 = BMI 30 = kg·m-2
Exclusion criteria
Exclusion criteria: SmokingSports/exercise >4 sessions/week or a structured resistance or endurance training scheduleLactose intolerant or allergies to milk proteinA history of neuromuscular problemsUse of anticoagulation medicationIndividuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription acne medications).Strict vegetarianInjury or condition that would limit the participant from performing the resistance exercise.PregnancyHormone replacement therapyBlood donation within the past 2 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| myofibrillar protein synthesis rates in the rested leg (integrative value over 0-24 h) | — |
Secondary
| Measure | Time frame |
|---|---|
| myofibrillar protein synthesis rates in the exercised leg (integrative value over 0-24 h) Other endpoints are, plasma glucose, plasma insulin, plasma amino acids concentrations, and plasma phenylalanine enrichments. | — |
Countries
Netherlands
Contacts
Public ContactJ Kaiser
Universiteit Maastricht
Outcome results
None listed