Digestion, absorption, amino acids, protein
Conditions
Interventions
Six protein blends with a 20g protein load in different protein ratios and volumes: MPW protein, native whey protein, MPW:MCI (40:60% ratio) protein, heat-treated MPW:MCI (40:60% ratio), heat-treated
Sponsors
FrieslandCampina (FC C.V.)
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Apparently healthy;Age 50 years and older;Body mass index (BMI) =18.5 and =30 kg/m2 ;Having veins suitable for blood sampling via a catheter (judged by study nurse/ medical doctor).Willing to comply with study proceduresAnd available for the 6 weeks of the study
Exclusion criteria
Exclusion criteria: Any self-reported metabolic, gastrointestinal, inflammatory or chronic disease (such as anemia, diabetes, hepatitis, cardiovascular disease);Having a history of medical or surgical events that may significantly affect the study outcome (judged by medical doctor), including: Inflammatory bowel disease, hepatitis, pancreatitis, ulcers, gastrointestinal or rectal bleeding; major gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel resection; known or suspected gastrointestinal disorders, colon or GI tract cancer;Use of medication that may impact gastric emptying (e.g. gastric acid inhibitors or laxatives) or glucose metabolism;Anaemia (Haemoglobin (Hb) values <7.5 mmol/L for women and <8.5 mmol/L for men), as assessed by finger prick blood during screening visit;Having a cow’s milk allergy and/or being lactose intolerant (self-reported);Use of antibiotic treatment less than 1 month before start of the study and during the study;Use of protein supplements (must be stopped 1 week before the first test day); Reported weight loss or weight gain of > 3 kg in the month prior to pre-study screening, or intention to lose weight during the study period;Reporting to follow or having planned a slimming or medically prescribed diet.Not willing to keep a stable lifestyle during the study period (e.g. planned change in diet or exercise during the study period);Recent blood donation (<1 month prior to test day 1 of the study) or not willing to stop donation during and 1 month after the study;Average alcohol intake >14 (women) or >21 (men) of alcoholic units per week;Abuse of illicit drugs; Current smokers; Not having a general practitioner; Insufficient proficiency in Dutch to understand information brochure and questionnaires;Participation in any clinical trial including blood sampling and/or administration of substances up to 30 days before test day 1 of this study and during the study period;Being an employee of the department Food, Health & Consumer Research of Wageningen Food & Biobased Research or FrieslandCampina R&D.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Postprandial plasma amino acid profiles of 20 free amino acids measured in blood samples collected before and up to 5 hours after the protein load. | — |
Secondary
| Measure | Time frame |
|---|---|
| Postprandial plasma amino acid profiles of 20 free amino acids measured in blood samples collected before and up to 5 hours after the protein load. | — |
Countries
Netherlands
Contacts
Public ContactM Viskaal
FrieslandCampina (FC C.V.)
Outcome results
None listed