Ischaemia reperfusion injury cardiac damage
Conditions
Interventions
A single session of local handgrip exercise with an intensity of 50% of their maximal contractile strength.
Sponsors
Radboud Universitair Medisch Centrum
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Adults >18 years, <65 years. - BMI < 30kg/m2 - Recreational athlete (i.e. minimal 1h/week exercise, maximal 5 days/week of exercise) - Mentally able/allowed to give informed consent
Exclusion criteria
Exclusion criteria: A potential subject who meets any of the following criteria will be excluded from participation in this study: - Presence of an absolute contra-indication for the performance of exercise (see also SOP Inspanningstest Department of Physiology): - Acute myocardial infarction (3-5 days) - Unstable angina - Uncontrolled arrhythmias causing symptoms or hemodynamic compromise - Active endocarditis - Acute myocarditis or pericarditis - Symptomatic severe aortic stenosis - Uncontrolled heart failure - Acute pulmonary embolus or pulmonary infarction - Thrombosis of lower extremities - Suspected dissecting aneurysm - Uncontrolled asthma - Pulmonary oedema - Respiratory failure - Acute noncardiopulmonary disorder that may affect exercise performance or be aggravated by exercise (i.e. infection, renal failure, thyrotoxicosis)- Smoking - Mental impairment leading to inability to cooperate - Use of medication that alters the effect of cardioprotection: • ß-blockers • Calcium Channel blockers • Nitrates • Opioids • Anti-platelet agents (e.g. paracetamol) • Statins and anti-hyperlipidaemic drugs • Anti-diabetic treatment
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The reduction of myocardial injury via the transfer of humoral cardioprotective agents, present in plasma obtained before/after handgrip exercise in various ex vivo models of IR. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary study parameters involve the measurement of cardiac mechanical function, altered cardioprotective signal transduction, histological assessment of tissue integrity, metabolic alterations, and the detection of biomarkers of injury (lactate dehydrogenase). | — |
Countries
Netherlands
Contacts
Public ContactTom Luiken
Radboud Universitair Medisch Centrum
Outcome results
None listed