coronary artery disease myocardial perfusion
Conditions
Interventions
None listed
Sponsors
University Medical Center Groningen
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: o Healthy subjects: * Age above 40 years * Gender: 50% women, 50% men * Good general health * BMI
Exclusion criteria
Exclusion criteria: Healthy subjects: * Clinical history of diabetes, hypertension, dyslipidemia * History of cardiac disease * Family history of Coronary Artery Disease * Active or short history of cigarette smoking * Inability or unwillingness to give informed consent * Known hypersensitivity to adenosine * Clinical active bronchospasm * Chronic obstructive pulmonary disease or asthma * Chronic kidney disease * Unwillingness to continue with the protocol * Pregnancy or breastfeeding * Claustrophobia o Patients recently diagnosed with CAD: * Known hypersensitivity to adenosine * Bundle branch block * Presence or temporal or definitive pacemaker * Presence of heart valve prosthesis, shoulder prosthesis, prosthetic reconstruction of the chest wall, or other prosthetic devices within the field of view * History of cardiovascular intervention, percutaneous coronary intervention (PCI), and coronary artery bypass grafting (CABG) * Clinical active bronchospasm * Chronic obstructive pulmonary disease or asthma * Unwillingness to continue with the protocol * Pregnancy or breastfeeding * Claustrophobia
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main parameters to measure are regional and global MBF and CFR during rest and stress with the proposed stressor: adenosine, and breath-holding maneuver. MBF will be calculated by dedicated software (MBF Siemens) in ml/g/min based on [13N]NH3 Total-body PET/CT acquisitions. CFR will be calculated as the ratio of stress MBF and rest MBF. CFR=(stress MBF)/(rest MBF) Data of myocardial perfusion will be analysed to address the feasibility of breath-holding maneuver as a stressor for myocardial perfusion test during [13N]NH3 Total-body PET/CT. Adenosine stress serves as the reference method: MBF stress above 1.85 mL/min/g and CFR above 2.0.(27) Final values in these parameters will be reported for all the cohorts in a descriptive manner: -Normally distributed quantitative variables will be presented with mean, standard deviation and 95% confidence interval. -Non-normally distributed data will be presented with median and interquartile range. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary parameters will be measured by rest and stress phases (with adenosine stress and with the proposed stressor: breath-holding maneuver): • Left ventricular volumes are automatically calculated by a dedicated software (MBF Siemens) from ECG-gated total-body PET/CT reconstruction. End-systolic volume (ESV) is the minimum point of the time-volume curve. End-diastolic volume (EDV) is the maximum point of the time-volume curve from the acquisition. Left ventricular ejection fraction is calculated automatically as follows: %LVEF=(EDV - ESV)/EDV x 100. • CBF is the blood volume that flows per unit per mass per unit in time in brain tissue calculated by software (PMOD) and expressed in ml/g/min based on [13N]NH3 Total-body PET/CT acquisitions. • RBF and SBF refer to the blood volume that flows to the kidneys and to the spleen respectively. These measurements are expressed in ml/g/min and will be calculated using dedicated software (PMOD) based on [13N]NH3 PET/CT dynamic images. Quantitative descriptive analysis will be performed to report the performance of CBF, RBF, and SBF in rest and during physiological and adenosine stress. | — |
Countries
Netherlands
Outcome results
None listed