Assessment of myocardial susceptibility to ischemia-reperfusion injury following marathon running.

Assessment of myocardial susceptibility to ischemia-reperfusion injury following marathon running. - Marathon and IRI injury

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON57441

Enrollment

Registered

2025-04-15

Start date

2025-10-03

Completion date

Unknown

Last updated

2026-03-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Ischemia-reperfusion vascular damage

Interventions

None listed

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: - Healthy male volunteers, adults >18 years,

Exclusion criteria

Exclusion criteria: A potential subject who meets any of the following criteria will be excluded from participation in this study: - Presence of an absolute contra-indication for the performance of exercise. - Mental impairment leading to inability to cooperate - Participants are instructed to limit the amount of moderate to vigorous exercises 48 hours before the start of the marathon. This is to prevent a potential cardioprotective phenotype before the start of the study. Participants that have not adhered to the restrictions will be excluded from the study. - Participants will be excluded in case they do not complete the marathon.

Design outcomes

Primary

Measure	Time frame
The primary outcome parameter focuses on infarct size (= degree of cell death) introduced by global IR in our ex vivo model of myocardial infarction which is presened as a percentage of infarcted tissue relative to the total ventricular tissue.	—

Secondary

Measure	Time frame
Isolated rat Heart - The release of the damage marker Lactate Dehydrogenase (LDH) into the cardiac effluent during reperfusion due to cardiac damage. - The restoration of mechanical function of the heart. Before starting the experimental protocol, a balloon will be introduced into the left ventricle to measure left ventricular pressure. This allows changes in developed pressure before and after the introduction of ischemia to be examined. - Protein changes that can provide information about intracellular mechanism will be investigated by Western blot analysis. Alterations in adverse and cardioprotective signalling pathways will be considered. Participant blood samples - Participants are assessed on concentrations of markers of injury or stress in plasma (cardiac Troponin T/I, LDH and Creatine Kinase). Plasma samples are collected before, after, and 4 hours after completion of the marathon. Residual plasma is stored at -80 * for a maximum of 15 years for subsequent analysis.	—

Measure

Time frame

Isolated rat Heart - The release of the damage marker Lactate Dehydrogenase (LDH) into the cardiac effluent during reperfusion due to cardiac damage. - The restoration of mechanical function of the heart. Before starting the experimental protocol, a balloon will be introduced into the left ventricle to measure left ventricular pressure. This allows changes in developed pressure before and after the introduction of ischemia to be examined. - Protein changes that can provide information about intracellular mechanism will be investigated by Western blot analysis. Alterations in adverse and cardioprotective signalling pathways will be considered. Participant blood samples - Participants are assessed on concentrations of markers of injury or stress in plasma (cardiac Troponin T/I, LDH and Creatine Kinase). Plasma samples are collected before, after, and 4 hours after completion of the marathon. Residual plasma is stored at -80 * for a maximum of 15 years for subsequent analysis.

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Countries

Netherlands

Outcome results