The effect of repeated sessions of whole-body neuromuscular electrical stimulation (NMES), with or without subsequent intake of a protein bolus, on muscle protein synthesis in healthy, young volunteers during 3 days of bed rest.

The effect of repeated sessions of whole-body neuromuscular electrical stimulation (NMES), with or without subsequent intake of a protein bolus, on muscle protein synthesis in healthy, young volunteers during 3 days of bed rest. - SPRINT

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON57421

Enrollment

Registered

2025-04-02

Start date

2025-05-22

Completion date

Unknown

Last updated

2025-10-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Muscle loss muscle weakness

Interventions

3-day bed rest study in which participants receive sham NMES followed by a 20g glucose drink (CON), whole body NMES stimulation followed by a 20g glucose drink (WB-NMES), or whole body NMES followed

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: • Aged from 18-35 years • 18.5

Exclusion criteria

Exclusion criteria: • Employed or undertaking a thesis or internship at the department of Human and Animal Physiology at Wageningen University • Smoking • Diabetes (Type 1, Type 2, or genetic form of diabetes) • Any diagnosed cardiovascular (heart) disease or high blood pressure (>=140 mmHg systolic and/or >=90 mmHg diastolic) • Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices) • Known allergy to lidocaine • Prone to keloid forming (i.e. hyperplastic growth of scars) • All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis) • Regular use of dietary protein and/or amino acid supplements (>3 times per week) • Currently involved in a structured progressive resistance training programme (>3 times per week) • A personal or family history of thrombosis (clots), epilepsy, seizures, or schizophrenia • Any previous motor disorders or disorders in muscle and/or lipid metabolism • Any back, leg, knee, neck, shoulder or postural complaints • Presence of an ulcer in the stomach or gut and/or strong history of indigestion • Contra-indications for DXA scans (e.g. undergoing radiologic examination) • Lactose intolerance • Known severe kidney problems • Pregnant or breastfeeding • Unable to give consent

Design outcomes

Primary

Measure	Time frame
Myofibrillar fractional synthetic rate (muscle protein synthesis).	—

Secondary

Measure	Time frame
• Quadriceps muscle layer thickness (measured via ultrasound) • Body composition (measured by dual-energy X-ray absorptiometry; DXA) • Quadriceps muscle strength and function (measured via the Humac Norm Dynamometer) • Handgrip strength • 24h nitrogen balance (measured via dietary intake and urinary nitrogen excretion)	—

Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)