SARS-CoV-2 antibody-mediated inflammatory responses by macrophages in individuals with and without obesity

SARS-CoV-2 antibody-mediated inflammatory responses by macrophages in individuals with and without obesity - SAMO

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON57369

Enrollment

Registered

2025-03-31

Start date

2025-04-22

Completion date

Unknown

Last updated

2026-03-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

coronavirus COVID-19

Interventions

None listed

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: Cohort 1 (obesity cohort) - >=18 and =30 kg/m2 - Patient of Franciscus Hospital Cohort 2 (control cohort) - >=18 and

Exclusion criteria

Exclusion criteria: Cohort 1 and 2 (obesity cohort and control cohort) - Acute infection or current systemic immunological disorders - Use of immune-modulatory medication (i.e., corticosteroids and biologicals) - Hormonal therapy

Design outcomes

Primary

Measure	Time frame
Measurement of pro-inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF) production by macrophages in response to SARS-CoV-2 viral and antibody costimulation.	—

Secondary

Measure	Time frame
- Pro-inflammatory cytokine (IL-6, IL-1β, and TNF) production by macrophages upon separate viral stimulation and antibody stimulation - Macrophage expression levels of activation markers and Fc-gamma receptors - Macrophage intracellular lipid content - Macrophage metabolic profiling - mRNA expression of proteins of interest in macrophages - Epigenetic analyses: chromatin accessibility, histone modification, and transcription/epigenetic factor binding - Proteomics (profiling of plasma proteins) - SARS-CoV-2 antigen-specific T cell responses - SARS-CoV-2 antigen-specific antibodies - Clinical chemistry and haematology data (only cohort 1) - Plasma pro-inflammatory cytokine levels - Additional cytokines and chemokines produced by macrophages upon stimulation with antibodies - History of and severity of prior SARS-CoV-2 infections	—

Measure

Time frame

- Pro-inflammatory cytokine (IL-6, IL-1β, and TNF) production by macrophages upon separate viral stimulation and antibody stimulation - Macrophage expression levels of activation markers and Fc-gamma receptors - Macrophage intracellular lipid content - Macrophage metabolic profiling - mRNA expression of proteins of interest in macrophages - Epigenetic analyses: chromatin accessibility, histone modification, and transcription/epigenetic factor binding - Proteomics (profiling of plasma proteins) - SARS-CoV-2 antigen-specific T cell responses - SARS-CoV-2 antigen-specific antibodies - Clinical chemistry and haematology data (only cohort 1) - Plasma pro-inflammatory cytokine levels - Additional cytokines and chemokines produced by macrophages upon stimulation with antibodies - History of and severity of prior SARS-CoV-2 infections

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Countries

Netherlands

Outcome results