the development of blood clots in the veins after vaccination Vaccine-induced Immune Thrombotic Thrombocytopenia (VITT)
Conditions
Interventions
None listed
Sponsors
Leids Universitair Medisch Centrum
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Age 18 years or older - Ability to understand the patient information form and ability to provide written informed consent. The criteria used for allocation of patients to their specific study group are as follows: Criteria specific to VITT patients: - Diagnosed with VITT based on diagnostic laboratory testing (performed by Sanquin) after vaccination with ChAdOx1 nCoV-19 or Ad26.COV2.S Criteria specific to aHIT/HIT patients: - Diagnosed with HIT following exposure to heparin or with spontaneous HIT/aHIT. Diagnosis based on diagnostic laboratory testing (performed by Sanquin).
Exclusion criteria
Exclusion criteria: - Individuals who fail to meet the inclusion criteria. - Individuals who have donated 50 ml of blood (or more) less than two weeks prior to the respective time-point for any reason (such as routine clinical care, participation in another study, blood donations for the blood bank, etc.) - Individuals with a self-reported or otherwise known diagnosis of severe anemia (blood hemoglobine
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Isolation of PF-4-specific B cells from peripheral blood, determination of PF-4 reactive B cell receptor sequences/repertoires, generation of monoclonal, PF-4 specific antibodies, and phenotypic characterization of the PF4-specific B cell response. | — |
Secondary
| Measure | Time frame |
|---|---|
| n.a. | — |
Countries
Netherlands
Outcome results
None listed