Viral diseases, Viral infectious diseases
Conditions
Interventions
Exposure to a finely dispersed spray of aerosols containing 10% fluorescein
sodium in a 5 mL water solution, without and while wearing an FFP2 respirator
or surgical mask.
Sponsors
Radboud Universiteit Nijmegen
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: In order to be eligible to participate in this study, a human subject must meet all of the following criteria: - Healthy males and females - Aged 18-60 years at the time of inclusion - European (Caucasian) or Asian descent - No history of atopic disorders - No facial hair growth or shaved.
Exclusion criteria
Exclusion criteria: A potential human subject who meets any of the following criteria will be excluded from participation in this study: - Unable to complete the informed consent procedure independently - Individuals with medical and/or cosmetic surgery that altered the form of their face - Individuals who are medically unfit to wear a standard face mask (e.g., due to airway diseases such as COPD or asthma, or skin reaction) - Individuals with a history of exposure to fluorescein. This includes intravenous administration of fluorescein for imaging purposes (such as fluorescein angiography) or local administration of fluorescein in the eye for corneal damage diagnostics - Pregnancy or (partner) intention to become pregnant during the study period - Male with facial hair growth unwilling to shave it (beards and/or moustaches).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Total inward leakage, expressed as a percentage of the dose, based on the recovery of fluorescein tracer in saliva and urine following exposure to finely dispersed spray of inhalable aerosols with fluorescein dissolved in water. | — |
Secondary
| Measure | Time frame |
|---|---|
| Total inward leakage, expressed as a percentage of the dose, is based on the recovery of fluorescein tracer in the mask filters and skin wipes following exposure to finely dispersed spray of inhalable aerosols with fluorescein dissolved in water. The influence of facial hair length on total inward leakage in males will be analyzed by comparing leakage between different beard lengths (0 mm, 5 mm and 10 mm). | — |
Countries
Netherlands
Contacts
Public ContactK Lassing
Radboud Universiteit Nijmegen
Outcome results
None listed