Insulin sensitivity, muscle loss, muscle disuse
Conditions
Interventions
Participants with and without T2D will undergo 2 days of forearm immobilization
as a model of local physical inactivity. Before, throughout, and after
immobilization arteriovenous forearm balance me
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Males and females with or without diagnosed type 2 diabetesUse lifestyle changes, metformin, gliclazide, or a combination thereof as oral glucose-lowering treatments for T2DAged 18-80 years at the time of signing informed consent18.5 = BMI = 35 kg·m2
Exclusion criteria
Exclusion criteria: Type 1 or a genetic form of diabetes Any diagnosed cardiovascular (heart) disease or high blood pressure (=160 mmHg systolic and/or =100 mmHg diastolic) Chronic use of any prescribed or over-the-counter pharmaceuticals (excluding oral contraceptives and contraceptive devices) that interact with muscle substrate metabolism (e.g. selective serotonin reuptake inhibitors) Consumption of a low-carbohydrate dietSmoking or chewing tobacco Known anaemia Regular use of dietary protein and/or amino acid supplements (>3 times per week) Currently involved in a structured progressive resistance training program (>3 times per week) A personal or family history of thrombosis (clots) Any previous motor disorders or inborn errors in muscle and/or lipid metabolism History of kidney disease History of liver disease Pregnant or breastfeeding History of any drug or alcohol abuse in the past two years Claustrophobia Unable to give consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary study endpoint is the change in postprandial muscle insulin sensitivity from 0 to 48 hours, measured as the delta forearm glucose uptake (FGU) via the arteriovenous forearm balance technique. | — |
Secondary
| Measure | Time frame |
|---|---|
| Postprandial muscle insulin sensitivity, measured as the temporal response of forearm glucose uptake (FGU) via arteriovenous forearm balance.Forearm amino acid net balance and kinetics (i.e. the rate of appearance and disappearance) measured via the forearm balance technique and stable isotope tracer infusion.Whole-body substrate oxidation (measured via indirect calorimetry). | — |
Countries
Netherlands
Contacts
Public ContactM.L. Dirks
Wageningen Universiteit
Outcome results
None listed