Functional analysis of molecular mechanisms underlying parasympathetic regulation of human adipose

Functional analysis of molecular mechanisms underlying parasympathetic regulation of human adipose - Parasympathetic regulation of adipose tissue

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON56908

Enrollment

Registered

2010-02-25

Start date

2010-10-10

Completion date

Unknown

Last updated

2024-08-19

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

diabetes Obesitas

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Patients referred for open elective abdominal surgery for hernia or gall stones which are healthy except for surgical diagnosis

Exclusion criteria

Exclusion criteria: Any significant infectious disease Any significant chronic disease Minors aged under18 y Incapacitated adults

Design outcomes

Primary

Measure	Time frame
The adipose tissue specimens will be used for the determination of the following parameters: mRNA measurements by real time PCR, protein identification by western blot and immunohistochemistry, glycerol release (lipolysis) and cytokine release from isolated adipocytes, adipocyte size, cell type identification in adipose tissue by FACS analysis, signal transduction analysis in isolated adipocytes using specific agonists and antagonists in vitro.	—

Measure

Time frame

The adipose tissue specimens will be used for the determination of the following parameters: mRNA measurements by real time PCR, protein identification by western blot and immunohistochemistry, glycerol release (lipolysis) and cytokine release from isolated adipocytes, adipocyte size, cell type identification in adipose tissue by FACS analysis, signal transduction analysis in isolated adipocytes using specific agonists and antagonists in vitro.

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Countries

Netherlands

Outcome results