asthma cystic fibrosis wheeze
Conditions
Interventions
None listed
Sponsors
KIndergeneeskunde/ Kinderlongziekten en -allergologie
Eligibility
Age
No minimum to 17 Years
Inclusion criteria
Inclusion criteria: 1. age 1 year to 18 years 2. Need for a bronchoscopy based on clinical indications during office hours.
Exclusion criteria
Exclusion criteria: 1. All patients aged 1-18 years of age who suffer from a malignancy and also have an infectious disease, that are in need for diagnostic bronchoscopy. 2. Children that have undergone lung transplantation. 3. Children who are unstable during the procedure, where prolonging the duration will cause increased risks, as judged by the anaesthesiologist and paediatric pulmonologist performing de bronchoscopy. 4. Children admitted at the ICU 5. Children who are in need of an emergency bronchoscopy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. To establish epithelial organoid cell cultures for pediatric respiratory research to enable future mechanistic and in vitro intervention studies. 2: To compare gene expression profiles of cultured bronchial and nasal epithelial organoids (derived from brushings and BALF) with bronchial epithelial cells directly obtained from bronchial biopsies. 3: To compare gene expression profiles of nasal and bronchial brushings in children 4. To establish a biobank for pediatric airway samples for future research questions that may arise | — |
Secondary
| Measure | Time frame |
|---|---|
| none | — |
Countries
Netherlands
Outcome results
None listed