Exploration of the roles of N0, N1, and N2 neutrophils on bone and cartilage regeneration in vitro from blood samples drawn from healthy volunteers

Exploration of the roles of N0, N1, and N2 neutrophils on bone and cartilage regeneration in vitro from blood samples drawn from healthy volunteers - Neutrophils and Bone/Cartilage Regeneration

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON56745

Enrollment

Registered

2024-05-21

Start date

2024-07-01

Completion date

Unknown

Last updated

2024-12-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cartilage damage Fracture healing

Interventions

None listed

Eligibility

Age

18 Years to 64 Years

Inclusion criteria

Inclusion criteria: Healthy men and women, aged > 18 years Signed written consent to take part in the study

Exclusion criteria

Exclusion criteria: Current infection, such as: cold, flu, sore throat, cold sores, gastrointestinal infections, or any other infections. Recent tattoo or body piercing within last six months Minor dental surgery within 24 hours, or major dental surgery within one month Recent anemia. Travel to areas where mosquito-borne infections diseases are prevalent, such as malaria, dengue fever, and Zika virus infection Positive for HIV Ever injected recreational drugs Pregnancy and breastfeeding Have a history of cancer

Design outcomes

Primary

Measure	Time frame
The main study parameter involves the unique surface biomarkers and cytokines present in the medium of N1 and N2 neutrophils, which will be assessed using flow cytometry and ELISA. Additionally, the diverse stimulatory effects of N0, N1, and N2 neutrophils on the osteogenesis of BMSCs and the chondrogenesis of chondrocytes will be investigated through presto blue, ALP activity, qPCR, as well as alizarin red and alcian blue staining.	—

Measure

Time frame

The main study parameter involves the unique surface biomarkers and cytokines present in the medium of N1 and N2 neutrophils, which will be assessed using flow cytometry and ELISA. Additionally, the diverse stimulatory effects of N0, N1, and N2 neutrophils on the osteogenesis of BMSCs and the chondrogenesis of chondrocytes will be investigated through presto blue, ALP activity, qPCR, as well as alizarin red and alcian blue staining.

—

Secondary

Measure	Time frame
The metabolites produced by N1 and N2 neutrophils and their spatial distribution will be examined using MALDI-MSI.	—

Countries

Netherlands

Outcome results