Anabolic resistance protein malabsorption
Conditions
Interventions
None listed
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Aged 18-35 years or 67+ years - Body mass index (BMI) 18.5-30 kg/m2 - Regular stool frequency ranging from 1-3 times/day
Exclusion criteria
Exclusion criteria: - History of cardiovascular, respiratory, gastrointestinal, urogenital, neurological, psychiatric, dermatologic, musculoskeletal, metabolic, endocrine, haematological, immunologic disorders, allergy, major surgery and/or laboratory assessments which might limit participation in or completion of the study protocol, interfere with the execution of the experiment, or potential influence the study outcomes (to be decided by the principal investigator and responsible physician) - Major abdominal surgery interfering with gastrointestinal function (upon judgement of the principal investigator and responsible physician) - Use of medication which limit participation in or completion of the study protocol, interferes with the execution of the experiment, or potential influences the study outcomes (to be decided by the principal investigator and responsible physician) including but not limited to chronic use of anticoagulants, regular use of laxatives 14 days prior to the study or during the study period, use of antibiotics 3 months prior to the study or during the study period - Use of supplementation (i.e. vitamin, pre- and probiotic supplementation, protein) within 14 days prior to testing - Administration of investigational drugs or participation in any scientific intervention study prior to or during the study period, which may interfere with this study (upon judgement of the principal investigator and responsible physician) - Specific diet (e.g. vegetarian, vegan, gluten free, no diary) within the study period - Planning to lose weight during the study period - Excessive alcohol consumption (defined as > 14 alcoholic consumptions per week) - Smoking - Drug use - Pregnancy - Donated blood three months prior to the test day - No given permission to register participation in electronic patient file at MUMC+ and to add records of gastroduodenoscopy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary study parameter is the mRNA expression of brush border peptidases and small peptide and amino acid transporters in duodenal mucosal cells. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary study parameters include protein expression levels of brush border peptidases and small peptide and amino acid transporters in duodenal mucosal cells, fecal microbial fermentation metabolites and duodenal mucosal protein synthesis rate. | — |
Countries
Netherlands
Outcome results
None listed