veroudering ageing senescence
Conditions
Interventions
Graded intakes of the limiting amino acid (lysine or methionine) in a reference
amino acid mixture or the test protein source (milk, sorghum or black beans)
will be consumed by the participants on d
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Gender: male - Age: 20-35 years or 65-80 years - Body Mass Index (BMI): 18.5 - 30.0 kg/m2 - Healthy as assessed with a questionnaire - Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) - Willing to comply with study procedures and use of data - Having given written informed consent
Exclusion criteria
Exclusion criteria: Chronic disease, for example: - Diabetes mellitus / being treated for high blood glucose - Severe cardiovascular disease (exception: hypertension) - Hepatic disease (e.g. hepatitis) - Renal disease - Cancer - Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding) - Pancreatitis History of medical or surgical events that may affect GI function, study outcomes or disease risk for participants, for example: - Bariatric surgery - Gastrointestinal tract surgery - Digestive tract disorder - Chewing problems - History of deep venous thrombosis without anti-coagulation medication Medicine use that interferes with, protein metabolism, GI function and the study outcomes, for example: - Glucose lowering drugs - Proton pump inhibitors - Laxatives Habits that interfere with the study outcomes: - Protein supplement use (current use or less than 2 weeks past use) - Smoking - Drug abuse - Alcohol consumption >21 units/week or >4/day - Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet - Moderate to high intense physical activity for more than 5 hours a week Other: - Self-reported allergy or intolerance to the tested products - Weight loss of more than 3 kg in the 3 months prior to study screening - Current participation in other research and
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 13C-enrichment in breath samples will be expressed in APE. CO2 production rate (VCO2) will be measured by indirect calorimetry and expressed in mL/min. The rate of 13CO2 production (F13CO2) will be calculated using these two measurements. The slope for the graded intakes of the limiting amino acid and the oxidation level will be constructed for the reference and the test proteins. The ratio between the reference and test protein will be calculated to represent metabolic availability of the limiting amino acid in each protein source. | — |
Countries
Netherlands
Outcome results
None listed