Healthy ageing Healthy ageing
Conditions
Interventions
None listed
Sponsors
Vrije Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: In order to be eligible to participate in this study, young sedentary participants must meet all of the following criteria: • Aged between 18-30 years • Male or female • Not currently engaging in any formal exercise training or competitive sports • No chronic health conditions likely to affect exercise tolerance or the physiological responses to exercise In order to be eligible to participate in this study, young trained participants must meet all of the following criteria: • Aged between 18-30 years • Male or female • Currently engaging in formal training (at least 3 times per week) in competitive endurance sports • No chronic health conditions likely to affect exercise tolerance or the physiological responses to exercise In order to be eligible to participate in this study, older sedentary participants must meet all of the following criteria: • Aged between 50-65 years • Male or female • Not currently engaging in any formal exercise training or competitive sports • No chronic health conditions likely to affect exercise tolerance or the physiological responses to exercise In order to be eligible to participate in this study, older trained participants must meet all of the following criteria: • Aged between 50-65 years • Male or female • Currently engaging in formal training (at least 3 times per week) in competitive endurance sports • No chronic health conditions likely to affect exercise tolerance or the physiological responses to exercise
Exclusion criteria
Exclusion criteria: • Age that falls outside of 18-30 years (young groups) or 50-65 years (middle-aged groups) • Inability to provide informed consent • History of claustrophobia • Ineligibility to perform the exercise test described in this study protocol or follow instructions • Contraindication for MRI (e.g. pacemaker, claustrophobia; see F1 vragenlijsten screening MRI Amsterdam ) • Being under investigation for non-diagnosed disease at the time of investigation • Risk factors for exercise testing registered by a Dutch version of the pre-participation questionnaire (American college of sports medicine and American Heart association). Possible risk factors will be discussed with a medical specialist or general practitioner before a subject can be included • BMI >30 due to adiposity, since this is known to cause difficulties in obtaining muscle biopsies and NIRS measurements • Vastus lateralis adipose tissue thickness >10 mm due to difficulties in obtaining high quality NIRS data • Pregnancy • Are current smokers or have been a regular smoker within the last 12 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary outcomes are: maximal oxygen uptake (V*O2max), muscle volume, muscle strength, muscle power, muscle diffusing capacity for oxygen (DmO2), and indicators of mitochondrial morphology. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary parameters can be categorised into the following groups of variables: exercise capacity, mitochondrial content and morphology, ex-vivo mitochondrial respiratory function, in vivo mitochondrial energetics, histology and immunohistochemistry, muscle morphological variables, mitochondrial dynamics proteins, and physical activity status. Further details are given in the protocol section 8.1.2. | — |
Countries
Netherlands
Outcome results
None listed