Quantification of leukocyte dynamics in bone marrow of healthy humans

Quantification of leukocyte dynamics in bone marrow of healthy humans - DYNAMO (leukocyte DYNAmics in huMan bOne marrow (DYNAMO)

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON56359

Enrollment

Registered

2021-03-11

Start date

2021-12-08

Completion date

Unknown

Last updated

2024-08-19

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

immunological memory maintenance of immune cells

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Elective hip or knee replacement surgery, older than 55 years of age

Exclusion criteria

Exclusion criteria: (Pathological) fracture as surgery indication, chronic infection, daily use of immuno-modulatory drugs

Design outcomes

Primary

Measure	Time frame
The average turnover of different immune cell subsets in bone marrow of healthy human adults during homeostasis.	—

Secondary

Measure	Time frame
- The average turnover and lifespan of antigen-specific T cell subsets in bone marrow and paired blood; - The phenotype of immune cells in bone marrow and paired blood based on flow cytometric markers (percentage positive and mean fluorescence intensity). - The function of immune cells in bone marrow and paired blood based on in vitro stimulation assays, e.g. in IFN-g or TNF-a production. - Concentration of factors in plasma and bone marrow and paired blood that can influence leukocyte dynamics, such as growth and survival factors, infection parameters, and viral factors. - Comparison of the T-cell receptor (TCR) repertoire between blood and bone marrow; - Determination of the phenotype of adaptive NK cells in blood and bone marrow using scATAC-seq.	—

Measure

Time frame

- The average turnover and lifespan of antigen-specific T cell subsets in bone marrow and paired blood; - The phenotype of immune cells in bone marrow and paired blood based on flow cytometric markers (percentage positive and mean fluorescence intensity). - The function of immune cells in bone marrow and paired blood based on in vitro stimulation assays, e.g. in IFN-g or TNF-a production. - Concentration of factors in plasma and bone marrow and paired blood that can influence leukocyte dynamics, such as growth and survival factors, infection parameters, and viral factors. - Comparison of the T-cell receptor (TCR) repertoire between blood and bone marrow; - Determination of the phenotype of adaptive NK cells in blood and bone marrow using scATAC-seq.

—

Countries

Netherlands

Outcome results