Muscle wasting Protein synthesis
Conditions
Interventions
A 5 day deuterated water dosing protocol will be conducted during ICU admission
to measure diurnal rates of muscle protein synthesis. Patients will receive
400ml of 70 mol% enriched deuterated water
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: 1) Age >18 5 days
Exclusion criteria
Exclusion criteria: 1) Coagulation disturbances (not including use of anti-coagulants in prophylactic dosages) 2) Contraindication to enteral infusion (e.g. due to GI-tract perforation) 3) Rhabdomyolysis 4) Renal dysfunction defined as a serum creatinine >171 *mol/L or a urine output of less than 500 ml/last 24 hours 5) Patients requiring chronic veno-venous hemofiltration 6) Patients on any form of extracorporeal life support (ECMO/ELS) 7) Pregnant patients or lactating with the intent to breastfeed 8) Previous participation in a 2H amino acid tracer study within the last year
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Primary study parameters are the fractional rates of muscle protein synthesis (%/day) using muscle protein-bound 2H-alanine enrichments, plasma 2H-alanine enrichments and intramuscular free 2H-alanine enrichment. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary study parameters include: body water 2H2O enrichment, plasma muscle-specific protein synthesis rates (virtual biopsy), transcriptional changes in genes involved in muscle protein synthesis and breakdown and plasma amino acid concentrations. | — |
Countries
Netherlands
Outcome results
None listed