To assess muscle loss and muscle mass regain during immobilisation and recovery muscle loss muscle regain
Conditions
Interventions
1 week of one-legged immobilisation and 2 weeks of recovery. During the entire
study period, a dietary supplement will be consumed twice daily.
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: 1) Male 2) Aged 18-35 y 3) BMI 18.5-30.0 kg/m2
Exclusion criteria
Exclusion criteria: (Family) history of thrombosis Lower limb, back or shoulder injuries (which may interfere with the use of crutches) Allergies to milk protein Participation in structured resistance exercise program All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g., arthritis, spasticity/rigidity, all neurological disorders and paralysis) Any medications known to (or may) affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications) Diagnosed metabolic, cardiovascular or intestinal disorders A history of neuromuscular problems Use of anti-coagulants Smoking (Family) history of Factor V Leiden or other known thrombophilia Any recent hospital admission/ major surgery diagnosed diabetes use of protein and/or fish-oil supplements participation in a 2H2O study in previous 6 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To determine the effect of protein hydrolysate supplementation on skeletal muscle mass (quadriceps muscle cross sectional area (CSA)) following 1 week of leg immobilisation. | — |
Secondary
| Measure | Time frame |
|---|---|
| To determine the effect of protein hydrolysate supplementation on maximal leg muscle strength (1RM), whole-leg muscle CSA, type I and II muscle fibre size, muscle protein synthesis rates, and muscle signalling and gene transcription responses after 1 week of immobilisation and after 2 weeks of recovery. | — |
Countries
Netherlands
Outcome results
None listed