COVID-19 patients with severe or fatal outcome have a pre-existing state of immune-aging; exploring determinants of disease

COVID-19 patients with severe or fatal outcome have a pre-existing state of immune-aging; exploring determinants of disease - Pre-existing immune-aging in COVID-19

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON55094

Enrollment

152

Registered

2020-06-18

Start date

2021-03-10

Completion date

Unknown

Last updated

2024-04-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

corona virus COVID-19

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Suspected of COVID-19 Age * 18 years Providing informed consent after reading patient information

Exclusion criteria

Exclusion criteria: Insufficient knowledge of Dutch No signed informed consent

Design outcomes

Primary

Measure	Time frame
Cellular immune parameters - Extensive immunophenotyping of T-lymphocyte maturation subsets, including recently thymic emigrants - Extensive immunophenotyping of B-lymphocyte maturation subsets - Extensive immunophenotyping of monocyte maturation subsets - SARS-CoV-2 specific CD8+ T cell response using immunodominant peptides that are shared between SARS-CoV and SARS-CoV-2. Molecular immune parameters - Immune repertoire (T-cell receptor and B-cell receptor) sequencing, including replication history - Gene expression profiles related to premature immunosenescence in monocytes - HLA-typing Serological immune parameters - Cytokines, for instance: CCL2/CCL3/CCL4/CCL7/CXCL10/FASL/G-CSF/Galectin-9/GM-CSF /IFN/IFN/ IL-1b/IL1Ra/IL-6/IL-7/IL-8/IL-10/Il-12/IL-18/sCD163/sCD206/sIL2R/sIL6R/TNF* - Autoantibodies, for instance: ANA/ANCA/ anti-thyroid/anti-adrenal Serological hormonal parameters Hormonal axis; pituitary-growth hormone axis, pituitary-thyroid axis, pituitary-adrenal axis, pituitary-thyroid axis and sex hormone axis Insulin/glucose ratio Lepthin-ghrelin-adipokines	—

Measure

Time frame

Cellular immune parameters - Extensive immunophenotyping of T-lymphocyte maturation subsets, including recently thymic emigrants - Extensive immunophenotyping of B-lymphocyte maturation subsets - Extensive immunophenotyping of monocyte maturation subsets - SARS-CoV-2 specific CD8+ T cell response using immunodominant peptides that are shared between SARS-CoV and SARS-CoV-2. Molecular immune parameters - Immune repertoire (T-cell receptor and B-cell receptor) sequencing, including replication history - Gene expression profiles related to premature immunosenescence in monocytes - HLA-typing Serological immune parameters - Cytokines, for instance: CCL2/CCL3/CCL4/CCL7/CXCL10/FASL/G-CSF/Galectin-9/GM-CSF /IFN*/IFN*/ IL-1b/IL1Ra/IL-6/IL-7/IL-8/IL-10/Il-12/IL-18/sCD163/sCD206/sIL2R/sIL6R/TNF* - Autoantibodies, for instance: ANA/ANCA/ anti-thyroid/anti-adrenal Serological hormonal parameters Hormonal axis; pituitary-growth hormone axis, pituitary-thyroid axis, pituitary-adrenal axis, pituitary-thyroid axis and sex hormone axis Insulin/glucose ratio Lepthin-ghrelin-adipokines

—

Secondary

Measure	Time frame
Additional genetic analysis Exome sequencing and gene expression profiles to analyze the involved pathways obtained from cytokine analysis, (for example IL-6 pathway associated genes and ACE-2 associated cytokine-receptors that are shed from the plasma membrane)	—

Countries

Netherlands

Outcome results