intestinal discomfort lactose intolerance
Conditions
Interventions
During the three consecutive intervention periods the study participants will
consume twice a day 12 grams of sugar. This dose will consist of different
ratios between beta-lactose and dextrose: twi
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: • Apparently healthy men and women • Asian ethnicity • Age between 18 and 50 years • Body mass index (BMI) between 18.5 and 30 kg/m2 • LNP genotype • Avoiding dietary lactose in habitual diet • Regular stool frequency (on average at least once every two days)
Exclusion criteria
Exclusion criteria: • Any metabolic, gastrointestinal, inflammatory or chronic disease (such as diabetes, anaemia, hepatitis, cardiovascular disease) • History of gastro-intestinal surgery or having (serious) gastrointestinal discomfort • Use of pre- and/or probiotics • Use of medication that may influence the study results, such as laxatives and lactase peparations (e.g. Kerutab). Use of medication will be judged by the medical supervisor • Having used antibiotics in the 6 months prior to the start of the study • Reported slimming or medically prescribed diet • Current smokers • Alcohol intake >=2 (women) or >=4 (men) glasses of alcoholic beverages per day • Pregnant or lactating (or having the wish to become pregnant during the study period, self-reported) • Abuse of illicit drugs • Having food allergies (no food intolerances) • Insufficient proficiency in English to understand information brochure and questionnaires • Participation in another clinical trial at the same time • Being an employee of the department Consumer Science & Health group of Wageningen Food & Biobased Research, Human Nutrition department of Wageningen University, or FrieslandCampina.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study parameter is the change in fecal microbiota based on shotgun metagenomic sequencing upon repetitive consumption of (an increasing dose of) lactose. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary study parameters are exhaled hydrogen with the hydrogen breath test, stool characteristics (such as pH, microbial lactase activity and dry weight), and GI comfort. | — |
Countries
Netherlands
Outcome results
None listed