ouderdom Aging
Conditions
Interventions
None listed
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: • Age 20 - 30y and 60 - 75y • BMI 18.5 - 25 kg/m2 (young adults) 20 - 30 kg/m2 (elderly) • Willing to fast overnight for 12 hours • Willing to give a blood sample • Having veins suitable for blood sampling (judged by study nurse/medical doctor) • Having a general practitioner • Signed informed consent
Exclusion criteria
Exclusion criteria: • Diagnosed with metabolic and/or inflammatory disease (e.g. diabetes, cardiovascular disease (with the exception of hypertension), arthritis, arthrosis, and auto-immune diseases) • Current diagnosis of cancer • Regular use of medication that interferes with immune function (e.g. corticosteroids, cytokine blockers) • Regular use of medication that may interfere with metabolism (e.g. metabolic inhibitors or activators) • Use of medication that interferes with immune function and metabolism at least one week preceding the study visit (e.g. NSAID, anti-histamines, corticosteroids) • More than 4kg weight loss over the last 4 months • Vaccination within 1 month preceding the study visit (e.g. immunization against influenza, pneumonia, and travel-related infections) • Donated blood within 2 months preceding the study visit • Pregnant, lactating or wishing to become pregnant in the period between the screening and study visit (self-reported) • Regular use of hard drugs and soft drugs (i.e. weekly use) and at least no use within 2 months preceding the study visit • Excessive alcohol use (i.e. >14 glasses per week) • Use of cigarettes and other tobacco products • Participation in another study that involves an intervention 3 months preceding the study visit • Members of the research team • Working, or doing an internship or thesis at the division *Human Nutrition and Health*, Wageningen University
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Cytokine and lactate production after ex-vivo exposure to pro-inflammatory stimuli. | — |
Secondary
| Measure | Time frame |
|---|---|
| ROS production, phagocytic capacity, glycolytic capacity, mitochondrial capacity, metabolic response upon inflammation, immunophenotyping, differentiation, transcriptomics, metabolomics, circulating metabolic and immunological parameters in plasma or serum. Other study parameters - BMI - Body composition - CRP - FFQ - Questionnaires | — |
Countries
Netherlands
Outcome results
None listed