Allergy, Hypersensitivity
Conditions
Interventions
Products that are being used:Nanoparticles: Liposomes with different compositions will be used as well as PLGA nanoparticles, both as an alternative for aluminum hydroxide. Loading of liposomes/nanopa
• Purified house dust mite allergens Der p 1 and Der p 2 and house dust mite extract
• Vitamin D3, retinoic acid and a TGFb mimetic peptide as candidate anti-inflammatory adjuvants
• Nanoparticles: li
• Antibodies against receptors on DCs, for more effective targeting to the right antigen-presenting cells.Sialyalted allergens: Der p 1, Der p 2, Ara h 1, Ara h 2, Ara h 3, Ara h 6, and sialylated ver
• For house dust mite: purified Der p 1 and Der p 2, and sialylated versions thereof
• For peanut: purified Ara h 1, Ara h
Sponsors
Amsterdam UMC
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria:Subjects with doctor-diagnosed allergic rhinitis or convincing history of allergic rhinitis and a positive SPT and/or serum IgE levels for HDM, cat or pollen allergens -Subjects with a doctor-diagnosed peanut allergy or a convincing history of peanut allergy and positive SPT and/or serum IgE levels for peanut allergensSubjects with a doctor-diagnosed cashew and/or pistachio allergy or a convincing history of cashew nut/pistachio allergy and positive SPT and/or serum IgE levels for cashew nut/pistachio allergens Healthy subjects, defined as not having allergic or non-allergic rhinitis, peanut allergy or other inflammatory non-communicable diseases such as rheumatoid arthritis, type 2 diabetes, celiac disease, colitis ulcerosa, Crohn*s disease, and multiple sclerosis.Age between 18-65Signed informed consent
Exclusion criteria
Exclusion criteria: Exclusion criteriaHistory of AIT (SCIT or SLIT) with any allergen within the past year of the time of blood donation.Ongoing AIT (SCIT or SLIT) with any allergen at the time of blood donation.Vaccination within one week before blood donation.Immunosuppressive or biological medication (e.g. IL-5, anti-IgE therapy) within the last six months prior to blood donation.Severe immune disorders (including auto-immune diseases) and/or diseases requiring immunosuppressive drugs.Active malignancies or any malignant disease during the previous 5 years.Active inflammation or infection at the time of blood donation.Use of systemic steroids within 4 weeks before the blood donation.Treatment with systemic and local ß-blockers.Volunteers who are students or employees of one of the participating research groups or 1st grade relatives or partners of the investigators.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Not applicable. Blood from patients will be used to isolate immune cells (PBMCs) and use them in fundamental in vitro experiments. The only value that will be determined directly from blood is the allergen-specific IgE titer to determine how severe the allergy from thet allergic patient is. | — |
Secondary
| Measure | Time frame |
|---|---|
| Not applicable. | — |
Countries
Netherlands
Contacts
Public ContactR. Ree
Amsterdam UMC
Outcome results
None listed