Brandwonden NETosis and thrombosis formation
Conditions
Interventions
None listed
Sponsors
Vrije Universiteit Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Patients: - >= 18 years - Acute burns - Competent and temporarily incompetent patients - Informed Consent by patient or the legal representative - Admitted to the Burn Center of the Red Cross Hospital in Beverwijk, who are expected to be admitted at least until the second measurement moment - No acute psychiatric disorders - Sufficient Dutch proficiency Healthy volunteers: - >= 18 years - Competent - Informed Consent - Sufficient Dutch proficiency
Exclusion criteria
Exclusion criteria: Patients: - Known underlying diseases: cancer, immune deficiency, hypertension, complement, thrombosis disorder. Healthy volunteers: - Pregnancy or lactating - Any medication except contraceptives - Known underlying diseases: cancer, immune deficiency, hypertension, complement, thrombosis disorder.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Burn wound severity will be determined at day of admission (day 0/1), day 2-5 post-burn and at day of surgery (if applicable) or day 10 - 20 post-burn by clinical evaluation, Laser Doppler Imaging (LDI) (not day 10 - 20), and (immuno)histochemical analyses in burn skin biopsies. In biopsies taken at the same time points the occurrence of intra-microvascular thrombi and NETosis will be quantified. In addition, in blood samples collected simultaneously with clinical routine lab up to day 30 post-burn, the levels of cfDNA/NETs, DNase1 and endogenous pdC1inh will be quantified. MBL serum levels will be examined in relation to bacterial presence and wound healing. From healthy volunteers only blood samples will be collected for the quantification of cfDNA/NETs, DNase1 and endogenous pdC1inh levels. | — |
Secondary
| Measure | Time frame |
|---|---|
| Correlations/associations between wound deepening, thrombosis/NETosis, levels of cfDNA/NETs, DNase1 and endogenous pdC1inh will be investigated. In addition, associations of thrombosis/NETosis with burn wound parameters and characteristics of the patients collected from the medical file e.g. wound size (% burned of TBSA), wound closure time, co-morbidities, inhalation trauma, graft take, length of stay, number of surgical procedures will also be investigated. | — |
Countries
Netherlands
Outcome results
None listed