How tissue interactions regulate human peri-implantation embryo development and the impact of aneuploidy: the HEYDAY study

How tissue interactions regulate human peri-implantation embryo development and the impact of aneuploidy: the HEYDAY study - HEYDAY Study

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON53979

Enrollment

1682

Registered

2022-12-07

Start date

2023-01-12

Completion date

Unknown

Last updated

2024-04-09

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

in vitro fertilisation miscarriage

Interventions

None listed

Eligibility

Inclusion criteria

Inclusion criteria: Eligible embryo donors to donate embryos for this study meet the following criteria: - The availability of surplus (poor quality) fresh or cryopreserved embryos; - Signed informed consent form to donate surplus embryos for research - Age >= 18 years

Exclusion criteria

Exclusion criteria: A potential subject who meets any of the following criteria will be excluded from participation in this study: - Informed consent form only signed by one of the gamete providers or intentional parents - No surplus embryos - Surplus embryos with excessive degeneration or fragmentation (>50%)

Design outcomes

Primary

Measure	Time frame
Outcome parameters include embryo morphology before and after culture, live staining for developmental markers, molecular karyotyping, RNA or protein expression of markers for lineage establishment and signalling pathway activation.	—

Secondary

Measure	Time frame
To establish the molecular interactions between trophoblast, hypoblast and epiblast lineages that regulate their developmental progression, we will combine findings from live imaging analysis, immunofluorescence analysis, single cell transcriptomics analysis and single-cell epigenetic profiles to identify timing and activity of pathways involved. To test these pathways and to explore candidate extraneous signals that can support pre-implantation development, functional assessment will be performed using the main study parameters as read outs.	—

Measure

Time frame

To establish the molecular interactions between trophoblast, hypoblast and epiblast lineages that regulate their developmental progression, we will combine findings from live imaging analysis, immunofluorescence analysis, single cell transcriptomics analysis and single-cell epigenetic profiles to identify timing and activity of pathways involved. To test these pathways and to explore candidate extraneous signals that can support pre-implantation development, functional assessment will be performed using the main study parameters as read outs.

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Countries

Netherlands

Outcome results