vertering en microbioom digestion microbial fermentation
Conditions
Interventions
8-days preconditioning mild ketogenic controlled diet followed by a high fat
shake challenge with a naso-intestinal catheter.
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Male or female adults - BMI 18.5-30 kg/m2 - Suitable veins for insertion of cannula
Exclusion criteria
Exclusion criteria: - Having a history of medical or surgical events that may either put the subject at risk because of participation in the study, or influence the results of the study, including diabetes mellitus, a swallowing disorder, gastrointestinal or liver disease, dyslipidemia, sleeping apnea, irritable bowel syndrome, renal failure, cancer, nose/throat diseases, gastric bypass surgery, use of anticoagulants; as determined by the medical supervisor; - Having a bleeding/coagulation disorder, including hemophilia, Von Willebrand disease, Bernard-Soulier, Glanzmann thrombasthenia or thrombocytopenia; - Use of antibiotics within 3 months of starting the study or planned during the study; - Use of medications known to interfere with gastro-intestinal function (e.g. gastric acid inhibitors), as determined by medical supervisor; - Use of pro- and prebiotic supplements within 4 weeks of starting the study; - Planning or scheduled to undergo magnetic resonance imaging (MRI) at any time during the course of the study - Currently following a very low carbohydrate (ketogenic) diet; - Alcohol consumption >21 glasses a week (women) or >28 glasses a week (men); - Pregnant, lactating or wishing to become pregnant in the period of the study (self-reported); - Not willing to give up blood donation during the study; - Food allergies or intolerances for products that we use in the study; - Current smokers; - Current users of soft and/or hard drugs; - Participation in another clinical trial at the same time; - Having fewer than three bowel movements per week (having constipation) - Being an employee of the Food, Health & Consumer Research group of Wageningen Food & Biobased Research or Human Nutrition and Health Department of Wageningen University.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary study parameters are the microbial-derived metabolites from linoleic acid and plant sterols after consumption of the high fat shake. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary study parameters include microbiota composition and transcriptomic activity. Other outcomes include inflammatory markers and ex-vivo analyses. | — |
Countries
Netherlands
Outcome results
None listed