Digestie &
Conditions
Interventions
All subjects will perform a single experiment where they ingest 40 g of
collagen protein peptides in 400 mL water. Before and after ingestion, blood
samples, muscle and skin biopsies will be taken a
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 64 Years
Inclusion criteria
Inclusion criteria: - Aged between 18-35 years - Healthy, recreationally active (participating in recreational sports activities >= 1 and
Exclusion criteria
Exclusion criteria: - Smoking - Musculoskeletal disorders - Metabolic disorders - Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications). - Chronic use of gastric acid suppressing medication or anti-coagulants - Unstable weight over the last three months - Diagnosed GI tract disorders or diseases - Blood donation in the past 2 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint will be the muscle connective tissue protein synthesis over the period before and after intake of collagen protein peptides, as determined using stable isotope tracer methodology. | — |
Secondary
| Measure | Time frame |
|---|---|
| To quantify the digestion and absorption kinetics of collagen peptides and the subsequent impact on skin connective tissue protein synthesis rates in vivo in humans. Also, incorporation of collagen-derived amino acids in muscle & skin tissue will be determined. Other secondary outcomes include whole-body protein kinetics (total rate of appearance, exogenous rate of appearance, endogenous rate of appearance, rate of disappearance), whole-body protein metabolism (synthesis, breakdown, oxidation, net balance), overall plasma amino acid responses, postprandial hydroxyproline concentration over time (iAUC) and plasma insulin responses. | — |
Countries
Netherlands
Outcome results
None listed